Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET)

The kit is developed for the binding of IgG Fc region to the human Fc gamma RIIA / CD32a (H167) receptor. It is used as a universal detection tool to identifying the ability of antibody drugs to bind to Fc gamma RIIA / CD32a (H167).

Blue ice

Human Fc gamma RIIA / CD32a (H167) binding kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). Use the mixture of biotinylated human Fc gamma RIIA / CD32a (H167) and Europium-chelate labeled streptavidin as the donor, FA labeled Human IgG1 antibody as the acceptor.
- In the absence of human Fc gamma RIIA/CD32a (H167) binding components, the donor and acceptor are in close proximity due to the binding of human Fc gamma RIIA/CD32a (H167) and FA-labeled Human IgG1 antibody. Under specific light source excitation, the donor emits a 620 nm signal, which is absorbed by the acceptor, resulting in a 665 nm emission.
- In the presence of human Fc gamma RIIA/CD32a (H167) binding components, they prevent the donor-acceptor interaction, thereby inhibiting FRET from occurring.

The Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET) is based on a homogeneous (no wash) competition TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to measure the interaction between human CD32a (H167) and antibody drug candidates or CD32a (H167) inhibitors. It is designed to facilitate the ADCC and ADCP functional performance evaluation of antibody drug candidates, high-throughput screening of CD32a (H167) inhibitors within 0.5-1 hours. It can also be used as a universal detection tool to identify the ability of antibody drugs to bind to human CD32a (H167).

1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.