PKCzeta Antibody, pAb, Rabbit

USA

GenScript Rabbit Anti-PKCζ, Polyclonal Antibody detects endogenous levels of total PKCζ, protein. No cross-reactivity to other PKC isoforms is detected.

Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. The PKC family has been divided into three groups differing in the enzymes' cofactor requirements: conventional (c)PKC isoforms (comprising alpha, betaI (also known as beta1), betaII (also known as beta2) and gamma)), that require diacylglycerol (DAG), Ca2+, and phospholipid for activation, novel (n)PKC isoforms (comprising delta, epsilon, eta (also known as PKC-L), theta and u (the mouse homolog of human PKCu, known as PKD)) that require DAG but not Ca2+, and atypical (a)PKC isoforms, namely zeta, iota and lambda (the mouse homolog of human PKC iota) that require neither Ca2+ nor DAG. A new PKC member has recently been discovered and is referred to as PKCny. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKCzeta (PKC zeta) exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. GenScript, Rabbit Anti-PKCzeta Polyclonal Antibody, is developed in rabbit using a synthetic peptide (KLH-coupled) derived from human PKCzeta.

Immunoaffinity chromatography