PKCny Antibody, pAb, Rabbit

USA

GenScript Rabbit Anti-PKCν, Polyclonal Antibody detects endogenous human and mouse PKCν, protein.

Protein kinase C (PKC, EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. It is involved in diverse cellular signaling pathways. Specifically, PKC family members serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC family is divided into three groups based on different enzymes cofactor requirements. They are conventional (c) PKC isoforms, novel (n) PKC isoforms, and atypical (a)PKC isoforms respectively. Conventional (c) PKC isoforms consisting, alpha, betaI, betaII, and gamma require diacylglycerol (DAG), Ca2+, and phospholipid for activation. Novel (n)PKC isoforms consisting delta, epsilon, eta (also known as PKC-L), theta and u(the mouse homolog of human PKCu, known as PKD) require DAG but not Ca2+ for activation. Atypical (a)PKC isoforms, consisting zeta, iota and lambda (the mouse homolog of human PKC iota) require neither Ca2+ nor DAG. Other than those listed above, a new PKC member has recently been discovered and is referred to as PKCny. PKCny (PKC nu), also known as Protein kinase D3 (PKD3), is widely expressed in human and murine tissue. It is stimulated by both phorbol ester and growth factors and is phosphorylated upon stimulation at Ser731 and Ser735, located within the activation loop. PKD3 continuously shuttles between the nucleus and the cytoplasm, which requires a functional CRM1 nuclear export pathway. GenScript, Rabbit Anti-PKCny Polyclonal Antibody, is developed in rabbit using a synthetic peptide (KLH-coupled) derived from human PKCny.

Immunoaffinity chromatography