« Back
Home /
Enterokinase, His, Bovine

Enterokinase, His, Bovine

Item no.	Z03004-500
Manufacturer	GenScript
Amount	500 IU
Quantity options	100 IU 500 IU 5000 IU
Category	Peptides & Proteins Recombinant Proteins
Type	Proteins
Specific against	Cattle (Bovine)
Purity	> 95% by SDS-PAGE analyses.
Dry ice	Yes
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z03004_500-Enterokinase_EK_Bovine,Enterokinase (EK), is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. Recombinant, Bovine Enterokinase (rbEK), as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques at GenScript.</td></tr><tr><th>M.W.</th><td colspan="7"> Theoretical MW: 22.7 kDa. Apparent MW: 40.0 kDa, observed by reducing SDS-PAGE.</td></tr><tr><th>Purity</th><td colspan="7"> > 95% by SDS-PAGE analyses.</td></tr><tr><th>Endotoxin Level</th><td colspan="7">< 1.0 EU/ug, determined by LAL method. </td></tr><tr><th>Storage</th><td colspan="7"> Recombinant Bovine Enterokinase (rbEK) remains stable up to 1 year at -20C from date of receipt. It will remain stable at 37C for one week without losing any activity.Please avoid freeze-thaw cycles.</td></tr><tr><th>Specificity</th><td colspan="7"> 5 IU/ul. Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 22C in a buffer containing 25mM Tris-HCl, pH 8.0.</td></tr><tr><th>Formulation</th><td colspan="7"> Sterile liquid solution contians 20mM Tris, 200mM NaCl, 2mM CaCl2, 50% glycerol, pH 7.4.</td></tr><tr><th>Note</th><td colspan="7"> For research use only</td></tr>
Similar products	Enterokinase(EK)
Shipping Condition	Dry ice
Available
Specificity	5 IU/ul. Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 22C in a buffer containing 25mM Tris-HCl, pH 8.0.
Manufacturer - Category	Proteins
Country of Origin	USA
Shipping Temperature	-20°C
Storage Conditions	-20°C
Molecular Weight	Theoretical MW: 22.7 kDa. Apparent MW: 40.0 kDa, observed by reducing SDS-PAGE.
Product Line	Enzymes
Manufacturer - Specificity	5 IU/ul. Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 22C in a buffer containing 25mM Tris-HCl, pH 8.0.
Description	Enterokinase (EK), is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. Recombinant, Bovine Enterokinase (rbEK), as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques at GenScript.
Formulation	Sterile liquid solution contians 20mM Tris, 200mM NaCl, 2mM CaCl2, 50% glycerol, pH 7.4.
Endotoxin Level	< 1.0 EU/ug, determined by LAL method.
Notes	For research use only

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Z03004-500-datasheet.pdf

Z03004-500-safety-datasheet.pdf