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CD40L/CD154/TRAP, Human

CD40L/CD154/TRAP, Human

Item no.	Z02022-10
Manufacturer	GenScript
Amount	10 ug
Quantity options	1 mg 10 ug 50 ug
Category	Oncology Food Safety Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis Aging & Neurological Disorders By Cell Type Carcinoma Peptides & Proteins Recombinant Proteins Cell Biology Cell Movement Cell Adhesion Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Cytoskeleton Proliferation Contaminants Metals By Organ Breast Cancer Heart & Blood Cancer small GTPases Rho Subfamily
Type	Proteins
Specific against	Human (Homo sapiens)
Purity	Greater than 95.0% as determined by: 1. Analysis by RP-HPLC. 2. Anion-exchange FPLC. 3. Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Citations	Schonbeck U, et al . Soluble CD40L and cardiovascular risk in women. Circulation. Nov 2001, 104(19): 2266-2268. Oertelt S, et al . Soluble CD40L in plasma of patients with primary biliary cirrhosis. Ann. N. Y. Acad. Sci. Jun 2005, 1051: 205-210. Danese S, et al . TNF-alpha blockade down-regulates the CD40/CD40L pathway in the mucosal microcirculation: a novel anti-inflammatory mechanism of infliximab in Crohn's disease. J. Immunol. Feb 2006, 176(4): 2617-2624.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02022_10-CD40_Ligand_CD40L_CD154_TRAP_Human,CD40 Ligand (CD40L/CD154/TRAP), is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. It also costimulates proliferation of activated T-cell and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. Recombinant Human CD40 Ligand (CD40L/CD154/TRAP), produced in, E. coli, is a non-glycosylated, polypeptide chain containing 149 amino acids and having a molecular mass of 16,308 Da.</td></tr><tr><th>M.W.</th><td colspan="7"> 16,308 Da</td></tr><tr><th>Purity</th><td colspan="7"> Greater than 95.0% as determined by: 1. Analysis by RP-HPLC. 2. Anion-exchange FPLC. 3. Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.1 ng/ug (1 EU/ug) of recombinant human CD40 Ligand (CD40L) as determined by LAL test.</td></tr><tr><th>Specific Activity</th><td colspan="7">Recombinant human CD40 Ligand (CD40L/CD154/TRAP), is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5-10 ng/ml, corresponding to a Specific Activity of 1.0 x 105 IU/mg.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized recombinant human CD40 Ligand (CD40L/CD154/TRAP), although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution CD40L should be stored at 4C between 2-7 days and for future use below -18C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).</td></tr><tr><th>Formulation</th><td colspan="7"> The protein was lyophilized after extensive dialysis against 50mM Tris-HCl, pH8.0, 250mM NaCl buffer.</td></tr><tr><th>Reconstitution</th><td colspan="7"> It is recommended to reconstitute the lyophilized recombinant human CD40 Ligand (CD40L/CD154/TRAP) in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.</td></tr><tr><th>Reference</th><td colspan="7">Schonbeck U, et al . Soluble CD40L and cardiovascular risk in women. Circulation. Nov 2001, 104(19): 2266-2268. Oertelt S, et al . Soluble CD40L in plasma of patients with primary biliary cirrhosis. Ann. N. Y. Acad. Sci. Jun 2005, 1051: 205-210. Danese S, et al . TNF-alpha blockade down-regulates the CD40/CD40L pathway in the mucosal microcirculation: a novel anti-inflammatory mechanism of infliximab in Crohn's disease. J. Immunol. Feb 2006, 176(4): 2617-2624.</td></tr>
Similar products	CD40
Shipping Condition	Cool pack
Available
Specificity	Recombinant human CD40 Ligand (CD40L/CD154/TRAP), is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5-10 ng/ml,
Manufacturer - Category	Proteins
Country of Origin	USA
Shipping Temperature	4°C
Storage Conditions	-80°C
Molecular Weight	16, 308 Da
Product Line	Immune Checkpoints
Manufacturer - Specificity	Recombinant human CD40 Ligand (CD40L/CD154/TRAP), is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5-10 ng/ml, corresponding to a Specific Activity of 1.0 x 105 IU/mg.
Description	CD40 Ligand (CD40L/CD154/TRAP), is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. It also costimulates proliferation of activated T-cell and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. Recombinant Human CD40 Ligand (CD40L/CD154/TRAP), produced in, E. coli, is a non-glycosylated, polypeptide chain containing 149 amino acids and having a molecular mass of 16, 308 Da.
Reconstitution	It is recommended to reconstitute the lyophilized recombinant human CD40 Ligand (CD40L/CD154/TRAP) in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Formulation	The protein was lyophilized after extensive dialysis against 50mM Tris-HCl, pH8.0, 250mM NaCl buffer.
Endotoxin Level	Less than 0.1 ng/ug (1 EU/ug) of recombinant human CD40 Ligand (CD40L) as determined by LAL test.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 10 ug

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