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TNF-α, His, Human

TNF-α, His, Human

Item no.	Z02685-50
Manufacturer	GenScript
Amount	50 ug
Quantity options	1 mg 10 ug 50 ug
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Glucose Homeostasis By Cell Type Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis
Type	Proteins
Specific against	Human (Homo sapiens)
Purity	> 95% by SDS-PAGE and HPLC analyses
Sequence	Val77-Leu233 (accession number: AAA72811.1), expressed with an N-terminal Met and 6xHis.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02685_50-TNF_alpha_Human_N_his_tagged,Tumor Necrosis Factor-Alpha (TNF-alpha), plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.Recombinant, human Tumor Necrosis Factor-Alpha (rhTNF-alpha), produced in E. coli is a non-glycosylated polypeptide chain of 164 amino acids. A fully biologically active molecule, rhTNF-alpha, has a molecular mass of 18.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.</td></tr><tr><th>M.W.</th><td colspan="7"> 18.3kDa, observed by reducing SDS-PAGE.</td></tr><tr><th>Purity</th><td colspan="7"> > 95% by SDS-PAGE and HPLC analyses</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> <1.0 EU/ug, determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> ED50 7.69 x 106units/mg.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized recombinant, human Tumor Necrosis Factor-Alpha (rhTNF-alpha), remains stable up to 12 months at -80C from date of receipt. Upon reconstitution, rhTNF-alpha should be stable up to 4 weeks at 4C or up to 6 months at -20C.</td></tr><tr><th>Formulation</th><td colspan="7">Lyophilized after extensive dialysis against PBS.</td></tr><tr><th>Reconstitution</th><td colspan="7"> Reconstituted in ddH2O at 100 ug/ml.</td></tr><tr><th>Sequence</th><td colspan="7"> Val77-Leu233 (accession number: AAA72811.1), expressed with an N-terminal Met and 6×His.</td></tr>
Similar products	TNF-alpha
Shipping Condition	Cool pack
Available
Specificity	ED50 7.69 x 106units/mg.
Manufacturer - Category	Proteins
Country of Origin	USA
Shipping Temperature	4°C
Storage Conditions	-80°C
Molecular Weight	18.3kDa, observed by reducing SDS-PAGE.
Product Line	Cytokines
Manufacturer - Specificity	ED50 7.69 x 106units/mg.
Description	Tumor Necrosis Factor-Alpha (TNF-alpha), plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. TNF alpha-1a is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.Recombinant, human Tumor Necrosis Factor-Alpha (rhTNF-alpha), produced in E. coli is a non-glycosylated polypeptide chain of 164 amino acids. A fully biologically active molecule, rhTNF-alpha, has a molecular mass of 18.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Reconstitution	Reconstituted in ddH2O at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against PBS.
Endotoxin Level	<1.0 EU/ug, determined by LAL method.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Z02685-50-datasheet.pdf

Z02685-50-safety-datasheet.pdf