Comparison

SCF, Human (P. pastoris-expressed) European Partner

Item no. Z02692-50
Manufacturer GenScript
Amount 50 ug
Quantity options 1 mg 10 ug 50 ug
Category
Type Proteins
Specific against Human (Homo sapiens)
Purity > 95% by SDS-PAGE and HPLC analyses.
Sequence Glu26-Ala189 (accession number: P21583.1), expressed with an N-terminal Met.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias protein/Z02692-Stem_Cell_Factor_SCF_human,Stem Cell Factor (SCF), is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant human Stem Cell Factor (rhSCF), produced in Pichia pastoris is a glycosylated polypeptide chain of 165 amino acids. A fully biologically active molecule, rhSCF has a molecular mass of ca.20kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.</td></tr><tr><th>M.W.</th><td colspan="7"> ca.20 kDa, observed by reducing SDS-PAGE.</td></tr><tr><th>Purity</th><td colspan="7"> > 95% by SDS-PAGE and HPLC analyses.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> <1.0 EU/ug, determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> The ED50, as determined by the dose-dependent stimulation of human TF-1 cells, is < 2 ng/ml, corresponding to a specific activity of 5×105 IU/mg. </td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized, recombinant human Stem Cell Factor (rhSCF), remains stable up to 12 months at -80C from date of receipt. Upon reconstitution, rhSCF should be stable up to 4 weeks at 4C or up to 6 months at -20C.</td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized after extensive dialysis against 10 mM acetic acid.</td></tr><tr><th>Reconstitution</th><td colspan="7"> Reconstituted in ddH2O at 100 ug/ml.</td></tr><tr><th>Sequence</th><td colspan="7"> Glu26-Ala189 (accession number: P21583.1), expressed with an N-terminal Met.</td></tr>
Similar products Stem
Shipping Condition Cool pack
Available
Specificity The ED50, as determined by the dose-dependent stimulation of human TF-1 cells, is < 2 ng/ml, corresponding to a specific activity of 5x105 IU/mg.
Manufacturer - Category
Proteins
Country of Origin
USA
Shipping Temperature
4°C
Storage Conditions
-80°C
Molecular Weight
ca.20 kDa, observed by reducing SDS-PAGE.
Product Line
Growth Factors
Manufacturer - Specificity
The ED50, as determined by the dose-dependent stimulation of human TF-1 cells, is < 2 ng/ml, corresponding to a specific activity of 5x105 IU/mg.
Description
Stem Cell Factor (SCF), is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant human Stem Cell Factor (rhSCF), produced in Pichia pastoris is a glycosylated polypeptide chain of 165 amino acids. A fully biologically active molecule, rhSCF has a molecular mass of ca.20kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
Reconstitution
Reconstituted in ddH2O at 100 ug/ml.
Formulation
Lyophilized after extensive dialysis against 10 mM acetic acid.
Endotoxin Level
<1.0 EU/ug, determined by LAL method.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 ug
Available: In stock
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