Manufacturer |
GenScript
|
Category |
|
Type |
Proteins |
Specific against |
Human |
Format |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Amount |
1mg |
Item no. |
Z02707-1 |
Targets |
SOD1, IL10 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Available |
|
Specificity |
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using murine MC/9-2 cells is less than 2 ng/ml, corresponding to a specific activity of >, 5.0 x 105 IU/mg. |
Description |
Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of ª-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 . IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts . Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells . IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer . The IL-10 dimer binds to two IL-10 Rª/IL-10R1 chains, resulting in recruitment of two IL-10 Rß/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 . IL-10Rß does not bind IL-10 by itself but is required for signal transduction . IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation . It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses. |
Endotoxin Level |
Less than 1 EU/ug of rHuIL-10 as determined by LAL method. |
Formulation |
Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4. |
M.W. |
Approximately 18.6kDa, a single non-glycosylated polypeptide chain containing 161 amino acids. |
Product Line |
Cytokine, Chemokines & Growth Factors |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 C. Further dilutions should be made in appropriate buffered solutions. |
Storage |
This lyophilized preparation is stable at 2-8 C, but should be kept at -20 C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 C to -70 C. Avoid repeated freeze/thaw cycles. |
Usage |
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
Product Origin |
USA |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.