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SPARC, Human

SPARC, Human

Item no.	Z02804-1
Manufacturer	GenScript
Amount	1 mg
Quantity options	1 mg 50 ug
Category	Oncology Neuroscience Neural Lineage Markers Neural Adhesion Molecules By Cell Type Carcinoma Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines Adenocarcinoma Cell Stainings ECM By Organ Bladder Cancer
Type	Proteins
Format	Sterile Filtered White lyophilized (freeze-dried) powder.
Specific against	Human (Homo sapiens)
Purity	>95% by SDS-PAGE and HPLC analyses.
Sequence	MSYYHHHHHH, PQQEALPDET, EVVEETVAEV, TEVSVGANPV, QVEVGEFDDG, AEETEEEVVA, ENPCQNHHCK, HGKVCELDEN, NTPMCVCQDP, TSCPAPIGEF, EKVCSNDNKT, FDSSCHFFAT, KCTLEGTKKG, HKLHLDYIGP, CKYIPPCLDS, ELTEFPLRMR, DWLKNVLVTL, YERDEDNNLL, TEKQKLRVKK, IHENEKRLEA, GDHPVELLAR, DFE
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02804-SPARC_Human, SPARC, an acronym for ‘secreted protein, acidic and rich in cysteine', is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid , 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis.</td></tr><tr><th>M.W.</th><td colspan="7"> Approximately 34.0 kDa, a single non-glycosylated polypeptide chain containing 286 amino acids, with expression vector sequence (containing 6 × His tag).</td></tr><tr><th>Purity</th><td colspan="7"> >95% by SDS-PAGE and HPLC analyses.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.2EU/ug of rHuSPARC as determined by LAL method.</td></tr><tr><th>Storage</th><td colspan="7"> This lyophilized preparation is stable at 2-8 C, but should be kept at -20 C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 C to -70 C. Avoid repeated freeze/thaw cycles.</td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.</td></tr><tr><th>Reconstitution</th><td colspan="7"> We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 C. Further dilutions should be made in appropriate buffered solutions.</td></tr><tr><th>Physical Appearance</th><td colspan="7"> Sterile Filtered White lyophilized (freeze-dried) powder.</td></tr><tr><th>Usage</th><td colspan="7"> This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.</td></tr><tr><th>Sequence</th><td colspan="7"> MSYYHHHHHH, PQQEALPDET, EVVEETVAEV, TEVSVGANPV, QVEVGEFDDG, AEETEEEVVA, ENPCQNHHCK, HGKVCELDEN, NTPMCVCQDP, TSCPAPIGEF, EKVCSNDNKT, FDSSCHFFAT, KCTLEGTKKG, HKLHLDYIGP, CKYIPPCLDS, ELTEFPLRMR, DWLKNVLVTL, YERDEDNNLL, TEKQKLRVKK, IHENEKRLEA, GDHPVELLAR, DFEKNYNMYI, FPVHWQFGQL, DQHPIDGYLS, HTELAPLRAP, LIPMEHCTTR, FFETCDLDND, KYIALDEWAGC, FGIKQKDIDK, DLVI</td></tr>
Similar products	SPARC
Shipping Condition	Cool pack
Available
Manufacturer - Category	Proteins
Country of Origin	USA
Shipping Temperature	4°C
Storage Conditions	-80°C
Molecular Weight	Approximately 34.0 kDa, a single non-glycosylated polypeptide chain containing 286 amino acids, with expression vector sequence (containing 6 x His tag).
Product Line	Other Proteins
Description	SPARC, an acronym for ‘secreted protein, acidic and rich in cysteine', is also known as osteonectin or BM-40. It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 303 amino acid , 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling. SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation. For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF. SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 C. Further dilutions should be made in appropriate buffered solutions.
Formulation	Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.
Endotoxin Level	Less than 0.2EU/ug of rHuSPARC as determined by LAL method.
Usage	This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Z02804-1.pdf

Z02804-1-datasheet.pdf

Z02804-1-safety-datasheet.pdf