« Back
Home /
Neurotrophin-4 (NT-4), Human

Neurotrophin-4 (NT-4), Human

Item no.	Z03000-50
Manufacturer	GenScript
Amount	50 ug
Quantity options	10 ug 50 ug
Category	Oncology Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Molecular Targets for Neuroscience Targets for Rodent Neurosciences Aging & Neurological Disorders Neural Signaling Infectious Diseases Viral Infections Coronavirus (COVID-19) Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Status Cell Metabolism Oxidative Stress By Organ Testicular & Prostate Cancer Neuronal Cell Markers
Type	Proteins
Specific against	Human (Homo sapiens)
Purity	> 95% by SDS-PAGE and HPLC analyses.
Sequence	Gly81-Ala210(accession number: P34130), expressed with an N-terminal Met.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z03000_50-Neurotrophin_4_NT_4_Human,Neurotrophin-4 (NT-4) , also known as NT-5, is a neurotrophic factor structurally related to beta-NGF, BDNF, and NT-3. Human NT-4 shares 48 - 52% aa sequence identity with human beta-NGF, BDNF, and NT-3.Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. NT-4 is expressed highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. NT-4 binds and induces receptor dimerization and activation of TrkB. NT-4 can signal through TrkB receptors and promotes the survival of peripheral sensory sympathetic neurons. Recombinant, human Neurotrophin-4 (rhNT-4), produced in, E.coli, is a noncovalently linked homodimer containing two non-glycosylated polypeptide chains of 131 amino acids. A fully biologically active molecule, rhNT-4 has a molecular mass of 28.1kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.</td></tr><tr><th>M.W.</th><td colspan="7"> 28.1 kDa, a noncovalently linked homodimer,of two 14.0 kDa polypeptide monomers.</td></tr><tr><th>Purity</th><td colspan="7"> > 95% by SDS-PAGE and HPLC analyses.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> < 0.3 EU/ug, determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> ED50, 2.0× 102 units/mg.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized recombinant, human Neurotrophin-4 (rhNT-4), remains stable up to 6 months at -80C from date of receipt. Upon reconstitution, rhNT-4 should be stable up to 2 weeks at 4C or up to 3 months at -20C.</td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized after extensive dialysis against 50mM acetic acid.</td></tr><tr><th>Reconstitution</th><td colspan="7"> Reconstituted in 50mM acetic acid or ddH2O at 50 ug/ml.</td></tr><tr><th>Sequence</th><td colspan="7"> Gly81-Ala210(accession number: P34130), expressed with an N-terminal Met.</td></tr><tr><th>Note</th><td colspan="7"> For research use only</td></tr>
Similar products	Neurotrophin-4
Available
Specificity	ED50, 2.0x 102 units/mg.
Country of Origin	USA
Storage Conditions	Lyophilized recombinant, human Neurotrophin-4 (rhNT-4), remains stable up to 6 months at -80C from date of receipt. Upon reconstitution, rhNT-4 should be stable up to 2 weeks at 4C or up to 3 months at -20C.
Molecular Weight	28.1 kDa, a noncovalently linked homodimer, of two 14.0 kDa polypeptide monomers.
Product Line	Cytokines, Chemokines & Growth Factors
Manufacturer - Specificity	ED50, 2.0x 102 units/mg.
Description	Neurotrophin-4 (NT-4) , also known as NT-5, is a neurotrophic factor structurally related to beta-NGF, BDNF, and NT-3. Human NT-4 shares 48 - 52% aa sequence identity with human beta-NGF, BDNF, and NT-3.Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. NT-4 is expressed highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. NT-4 binds and induces receptor dimerization and activation of TrkB. NT-4 can signal through TrkB receptors and promotes the survival of peripheral sensory sympathetic neurons. Recombinant, human Neurotrophin-4 (rhNT-4), produced in, E.coli, is a noncovalently linked homodimer containing two non-glycosylated polypeptide chains of 131 amino acids. A fully biologically active molecule, rhNT-4 has a molecular mass of 28.1kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Reconstitution	Reconstituted in 50mM acetic acid or ddH2O at 50 ug/ml.
Formulation	Lyophilized after extensive dialysis against 50mM acetic acid.
Endotoxin Level	< 0.3 EU/ug, determined by LAL method.
Notes	For research use only

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Z03000-50-datasheet.pdf

Z03000-50-safety-datasheet.pdf