LAG-1/CCL4L1, Human

USA

-80

Lymphocyte Activation Gene-1(Lag-1) , also known as C-C motif chemokine 4-like (CCL4L1), is a proinflammatory chemokine and is the isoform of Macrophage Inflammatory Protein-1 beta (MIP-1 beta). LAG-1 was first identified in 1988 and is expressed by monocytes, macrophages, T and B lymphocytes, and dendritic cells upon stimulation. The receptors of LAG-1 are the G-protein coupled receptors CCR1, CCR3, and CCR5 which is its major receptor. LAG-1 is a potent chemoattractant of monocytes, T lymphocytes, dendritic cells, natural killer cells, and activated CD4+ T cells. Unlike CCL4, LAG-1 cannot bind to glycosaminoglycans (GAGs), and thus cannot form an in vivo gradient on the cell surface as does CCL4. Like other members of the MIP-1 subfamily, LAG-1 is associated with several types of disease, including HIV infection and Type I diabetes.
Recombinant human LAG-1/CCL4L1 produced in E. coli is a single non-glycosylated polypeptide chain containing 69 amino acids. A fully biologically active molecule, rhLAG-1/CCL4L1 has a molecular mass of 7.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.