Melanoma Inhibitory Activity-2 (MIA-2), Human

USA

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Melanoma inhibitory activity protein 2(MIA-2) is a secreted cytokine that is highly expressed in the liver and weakly in the testis. It is a member of the MIA/OTOR family. Members of this family such as MIA, OTOR, and TANGO share a Src homology-3 (SH3)-like domain. Melanoma inhibitory activity 2 is mapped to the gene locus of human chromosome 14q13. MIA and OTOR are exclusively expressed in the cartilage and cochlea, respectively, whereas MIA-2 is expressed exclusively in the liver. MIA-2 expression is transcriptionally regulated by the hepatocyte nuclear factor (HNF)-1-binding site). It is expressed in hepatocellular carcinoma (HCC) but not in bladder, breast, or prostate cancer. MIA-2 inhibits HCC growth and invasion, and consequently acts as a tumor suppressor.
Recombinant Human MIA-2 produced in E. coli cells is a single non-glycosylated polypeptide chain containing 100 amino acids. A fully biologically active molecule, rhMIA-2 has a molecular mass of 11.6 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.