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TNF-α, Mouse (P. pastoris-expressed)

TNF-α, Mouse (P. pastoris-expressed)

Item no.	Z02918-20
Manufacturer	GenScript
Amount	20 ug
Quantity options	1 mg 100 ug 20 ug
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis By Cell Type Carcinoma Peptides & Proteins Recombinant Proteins Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Cardiovascular Research Ischemia & Reperfusion Injury Proliferation Hypertension
Type	Proteins
Specific against	Mouse (Murine, Mus musculus)
Purity	> 95% as analyzed by reducing SDS-PAGE.
Sequence	Ser84-Leu235 (accession number:P06804).
Citations	1. Friedmann, E. et al. (2006) Nat Cell Biol. 8: 843-48. 2. Pocsik, E. et al. (1995)JInflamm. 45: 152-60. 3. Hlodan, R. et al. (1995) Eur JBiochem. 231: 381-87. 4. Hehlgans, T. and K. Pfeffer (2005)Immunology 115:1-20.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02918_20-TNF_Mouse, Sharing 79% sequence identity with human Tumor necrosis factor-alpha (hTNF-alpha), mouse Tumor Necrosis Factor-alpha (mTNF-alpha), is a cytokine mainly expressed by immune cells [1]. A type II transmembrane protein, TNF-alpha is further proteolytically processed to a soluble form [2]. The trimeric active TNF-alpha[3] then exerts its diverse biological properties including gapoptosis, inflammation, autoimmunity and cell proliferation by binding to TNF Receptor 1 and 2 [4]. Recombinant, mouse Tumor Necrosis Factor-alpha (rmTNF-alpha), produced in, P.pastoris, is a fully biologically active polypeptide chain of 152 amino acids with a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.</td></tr><tr><th>M.W.</th><td colspan="7"> 17kDa, observed by reducing SDS-PAGE.</td></tr><tr><th>Purity</th><td colspan="7"> > 95% as analyzed by reducing SDS-PAGE.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> <1 EU/ug, determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> ED50 1 x 108units/mg.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized recombinant, mouse Tumor Necrosis Factor-alpha (rmTNF-alpha), remains stable up to 6 months at -80C from date of receipt. Upon reconstitution, rmTNF-alphashould be stable up to 1 week at 4C or up to 2 months at -20C.</td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized after extensive dialysis against PBS.</td></tr><tr><th>Reconstitution</th><td colspan="7"> Reconstituted in ddH2O or PBS at 100 ug/ml.</td></tr><tr><th>Sequence</th><td colspan="7"> Ser84-Leu235 (accession number:P06804).</td></tr><tr><th>Reference</th><td colspan="7">1. Friedmann, E. et al. (2006) Nat Cell Biol. 8: 843-48. 2. Pocsik, E. et al. (1995)JInflamm. 45: 152-60. 3. Hlodan, R. et al. (1995) Eur JBiochem. 231: 381-87. 4. Hehlgans, T. and K. Pfeffer (2005)Immunology 115:1-20.</td></tr>
Similar products	TNF-alpha
Shipping Condition	Cool pack
Available
Specificity	ED50 1 x 108units/mg.
Manufacturer - Category	Proteins
Country of Origin	USA
Shipping Temperature	4°C
Storage Conditions	-80°C
Molecular Weight	17kDa, observed by reducing SDS-PAGE.
Product Line	Cytokines
Manufacturer - Specificity	ED50 1 x 108units/mg.
Description	Sharing 79% sequence identity with human Tumor necrosis factor-alpha (hTNF-alpha), mouse Tumor Necrosis Factor-alpha (mTNF-alpha), is a cytokine mainly expressed by immune cells [1]. A type II transmembrane protein, TNF-alpha is further proteolytically processed to a soluble form [2]. The trimeric active TNF-alpha[3] then exerts its diverse biological properties including gapoptosis, inflammation, autoimmunity and cell proliferation by binding to TNF Receptor 1 and 2 [4]. Recombinant, mouse Tumor Necrosis Factor-alpha (rmTNF-alpha), produced in, P.pastoris, is a fully biologically active polypeptide chain of 152 amino acids with a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Reconstitution	Reconstituted in ddH2O or PBS at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against PBS.
Endotoxin Level	<1 EU/ug, determined by LAL method.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 20 ug

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