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PreScission Protease

PreScission Protease

Item no.	Z02799-250
Manufacturer	GenScript
Amount	250 IU
Quantity options	100 IU 250 IU 5000 IU
Category	Neuroscience Metabolism Diabetes Molecular Targets for Neuroscience Peptides & Proteins Recombinant Proteins
Type	Proteins
Format	Sterile liquid.
Applications	IS, SDS-PAGE
Specific against	other
Dry ice	Yes
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02799-PreScission_Protease_PSP, PreScission Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease . The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues . Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well.</td></tr><tr><th>Specific Activity</th><td colspan="7">Unit Definition: One unit is defined as the amount of enzyme needed to cleave 100 ug of fusion protein in 16 hours to 90% completion at 5C in a buffer containing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT. Cleavage Buffer: 50 mM Tris-HCl, pH 7.0 (at 25 C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5 C prior to use.</td></tr><tr><th>Storage</th><td colspan="7"> Should be stored in small aliquots at -20C to -80C for long term.</td></tr><tr><th>Applications</th><td colspan="7"> During cleavage reactions, it is recommended that samples be removed at various time points and analyzed by SDS-PAGE to estimate the yield, purity, and extent of digestion. The amount of PreScission Protease, temperature and length of incubation required for complete digestion of a given GST fusion partner may vary depending on the fusion partner. Optimal conditions for each fusion should be determined in pilot experiments. Digestion may be improved by adding TritonTM X-100, TweenTM 20, NonidetTM, or NP40 to a concentration of 0.01%. Concentrations of these detergents up to 1% do not inhibit PreScission Protease.</td></tr><tr><th>Physical Appearance</th><td colspan="7"> Sterile liquid.</td></tr><tr><th>Usage</th><td colspan="7"> This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE</td></tr><tr><th>Note</th><td colspan="7"> This material is offered for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Not available in the US or Canada.</td></tr>
Similar products	PreScission
Shipping Condition	Dry ice
Available
Specificity	Unit Definition: One unit is defined as the amount of enzyme needed to cleave 100 ug of fusion protein in 16 hours to 90% completion at 5C in a buffer containing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT. Cleavage Buffer: 50 mM Tris-HCl
Manufacturer - Category	Proteins
Country of Origin	USA
Shipping Temperature	-20°C
Storage Conditions	-20°C
Product Line	Enzymes
Manufacturer - Specificity	Unit Definition: One unit is defined as the amount of enzyme needed to cleave 100 ug of fusion protein in 16 hours to 90% completion at 5C in a buffer containing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT. Cleavage Buffer: 50 mM Tris-HCl, pH 7.0 (at 25 C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5 C prior to use.
Description	PreScission Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease . The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues . Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well.
Usage	This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE
Notes	This material is offered for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Not available in the US or Canada.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Z02799-250-datasheet.pdf

Z02799-250-safety-datasheet.pdf