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HB-EGF, Mouse

HB-EGF, Mouse

Item no.	Z02943-10
Manufacturer	GenScript
Amount	10 ug
Quantity options	1 mg 10 ug 50 ug
Category	Oncology Neuroscience Targets for Rodent Neurosciences Neural Signaling By Cell Type Carcinoma Hematology Myeloma Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines Cell Status Proliferation
Type	Proteins
Format	Sterile Filtered White lyophilized (freeze-dried) powder.
Specific against	Mouse (Murine, Mus musculus)
Purity	>97% by SDS-PAGE and HPLC analyses.
Sequence	DLEGTDLNLF KVAFSSKPQG LATPSKERNG KKKKKGKGLG KKRDPCLRKY KDYCIHGECR YLQEFRTPSC KCLPGYHGHR CHGLTL
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z02943_10-HB_EGF_Murine, Heparin-binding EGF-like growth factor (HB-EGF) is a member of the EGF family of proteins. HB-EGF-like growth factor is synthesized as a membrane-anchored mitogenic and chemotactic glycoprotein. An epidermal growth factor produced by monocytes and macrophages, due to an affinity for heparin is termed HB-EGF. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. The transmembrane form of HB-EGF is the unique receptor for diptheria toxin and functions in juxtacrine signaling in cells. Both forms of HB-EGF participate in normal physiological processes and in pathological processes including tumor progression and metastasis, organ hyperplasia, and atherosclerotic disease. HB-EGF can bind two locations on cell surfaces, heparan sulfate proteoglycans and EGF-receptor effecting cell to cell interactions.</td></tr><tr><th>M.W.</th><td colspan="7"> Approximately 9.8 KDa, a single non-glycosylated polypeptide chain containing 86 amino acids.</td></tr><tr><th>Purity</th><td colspan="7"> >97% by SDS-PAGE and HPLC analyses.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 1EU/ug of rMuHB-EGF as determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0 × 106 IU/mg.</td></tr><tr><th>Storage</th><td colspan="7"> This lyophilized preparation is stable at 2-8C, but should be kept at -20C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20C to -70C. Avoid repeated freeze/thaw cycles. </td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized from a 0.2 um filtered concentrated solution in 10mM PB, 500 mM NaCl, pH7.4.</td></tr><tr><th>Reconstitution</th><td colspan="7"> We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20C. Further dilutions should be made in appropriate buffered solutions.</td></tr><tr><th>Physical Appearance</th><td colspan="7"> Sterile Filtered White lyophilized (freeze-dried) powder.</td></tr><tr><th>Sequence</th><td colspan="7"> DLEGTDLNLF KVAFSSKPQG LATPSKERNG KKKKKGKGLG KKRDPCLRKY KDYCIHGECR YLQEFRTPSC KCLPGYHGHR CHGLTL</td></tr>
Similar products	HB-EGF
Shipping Condition	Cool pack
Available
Specificity	Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0 x 106 IU/mg.
Manufacturer - Category	Proteins
Country of Origin	USA
Shipping Temperature	4°C
Storage Conditions	-80°C
Molecular Weight	Approximately 9.8 KDa, a single non-glycosylated polypeptide chain containing 86 amino acids.
Product Line	Growth Factors
Manufacturer - Specificity	Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0 x 106 IU/mg.
Description	Heparin-binding EGF-like growth factor (HB-EGF) is a member of the EGF family of proteins. HB-EGF-like growth factor is synthesized as a membrane-anchored mitogenic and chemotactic glycoprotein. An epidermal growth factor produced by monocytes and macrophages, due to an affinity for heparin is termed HB-EGF. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. The transmembrane form of HB-EGF is the unique receptor for diptheria toxin and functions in juxtacrine signaling in cells. Both forms of HB-EGF participate in normal physiological processes and in pathological processes including tumor progression and metastasis, organ hyperplasia, and atherosclerotic disease. HB-EGF can bind two locations on cell surfaces, heparan sulfate proteoglycans and EGF-receptor effecting cell to cell interactions.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20C. Further dilutions should be made in appropriate buffered solutions.
Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 10mM PB, 500 mM NaCl, pH7.4.
Endotoxin Level	Less than 1EU/ug of rMuHB-EGF as determined by LAL method.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 10 ug

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Delivery expected until 9/11/2025

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