B7-H2/ICOSLG Fc Chimera, Human

USA

Lyophilized recombinant B7-H2 Fc Chimera, Human remains stable for up to 6 months at -80C from date of receipt. Upon reconstitution, Human B7-H2 Fc should be stable for up to 1 week at 4C or up to 3 months at -20C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

B7-H2, best known as the ligand of inducible co-stimulator, belongs to B7-CD28 family. B7-H2 is a transmembrane glycoprotein of approximately 60 kDa and is expressed on antigen presenting cells such as B cells, macrophages, dendritic cells, and also in monocytes. It’s a ligand for CD28 and CTLA-4 in human, whereas these interactions are not conserved in mouse. B7-H2 and B7-1 or B7-2 interacts with CD28 through distinctive domains. B7-H2-CD28 interaction is essential for the co-stimulation of human T cells' primary responses to allogeneic antigens and memory recall responses.
Recombinant Human B7-H2 Fc Chimera produced in HEK293 cells. It’s a polypeptide chain containing 473 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhB7-H2 has a molecular mass of 70-80 kDa, analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.