B7-H2/ICOSLG His, Human

USA

Lyophilized recombinant B7-H2/ICOSLG, His, Human remains stable up to 6 months at -80C from date of receipt. Upon reconstitution, Human B7-H2/ICOSLG should be stable up to 1 week at 4C or up to 3 months at -20C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

B7-H2, best known as the ligand of inducible costimulator, belongs to B7-CD28 family, is a transmembrane glycoprotein of approximately 60 kDa. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, dendritic cells, and also in monocytes, and is a ligand for CD28 and CTLA-4 in human, whereas these interactions are not conserved in mouse. B7-H2 and B7-1 or B7-2 interacted with CD28 through distinctive domains. B7-H2-CD28 interaction is essential for the costimulation of human T cells' primary responses to allogeneic antigens and memory recall responses.
Recombinant Human B7-H2/ICOSLG produced in HEK293 cells is a polypeptide chain containing 250 amino acids with C-terminal 10His. A fully biologically active molecule, rhB7-H2/ICOSLG has a molecular mass of 45-55 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.