SIRPalpha, His, Human

USA

Lyophilized recombinant SIRPalpha remains stable up to 6 months at -80C from date of receipt. Upon reconstitution; Human SIRPalpha should be stable up to 1 week at 4C or up to 3 months at -20C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Signal regulatory protein alpha (SIRPalpha, designated CD172a), is also known as CD172 antigen-like family member A (CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), which is a monomeric about 90kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily. SIRPalpha is Ubiquitous and highly expressed in brain. SIRPA/CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA/SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function by similarity. SIRPalpha recognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production.
Recombinant Human SIRPalpha produced in HEK293 cells is a polypeptide chain containing 346 amino acids with C-terminal 6His. A fully biologically active molecule, rhSIRPalpha has a molecular mass of 50-55 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.