MOUSE ANTI HUMAN B7-H4

B7-H4

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Recognises human B7-H4 also known as B7x a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7-H4 binds an unknown receptor it is thought to deliver an inhibitory signal to T-cells preventing their proliferation cell cycle progression and interleukin-2 production. B7-H4 deficient mice are only minimally affected; suggesting B7-H4 is important in the fine tuning of the T-cell mediated immune response. B7-H4 is expressed on activated T-cells B-cells monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung breast and ovary in humans. B7-H4 could be a useful prognostic marker in Renal Cell Carcinoma (RCC). Recommended Negative Controls: MOUSE IgG1 NEGATIVE CONTROLRecommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMHuCAL Anti Mouse IgG1Goat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L)

Expressed at the cell surface. A soluble form has also been detected. Ref. 12Ref. 1Ref. 3Tissue specificityOverexpressed in breast ovarian endometrial renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells monocytes and dendritic cells but not expressed in most normal tissues (at protein level). Widely expressed including in kidney liver lung ovary placenta spleen and testis. Ref. 12Ref. 13Ref. 17Ref. 1Ref. 3Ref. 11Ref. 15Induction: Up-regulated by IL-6 and IL-10 and inhibited by GM-CSF and IL-4 on antigen-presenting cells (APCs). Ref. 13Ref. 16Ref. 14Post-translational modificationN-glycosylated. Ref. 12Miscellaneous: May serve as a predictive marker for renal cell carcinoma. Ref. 15Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family. UniProtKB Q5ZPR3Contains 2 Ig-like V-type (immunoglobulin-like) domains. Caution: The mouse ortholog has been shown (PubMed:12818166) to be a GPI-anchored protein but the Gly residue which is predicted to be the modified site in mouse and rat is not conserved in human. [1] \" A human cDNA encoding the small subunit of RNA polymerase II transcription factor SIII. \" Garrett K. P. Haque D. Conaway R. C. Conaway J. W. Gene 150:413-414(1994) [PubMed: 7821821] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Peripheral blood. [2] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin. [3] \" Drosophila von Hippel-Lindau tumor suppressor complex possesses E3 ubiquitin ligase activity. \" Aso T. Yamazaki K. Aigaki T. Kitajima S. Biochem. Biophys. Res. Commun. 276:355-361(2000) [PubMed: 11006129] [Abstract]Cited for: INTERACTION WITH VHL. [4] \" Amplification and overexpression of Elongin C gene discovered in prostate cancer by cDNA microarrays. \" Porkka K. Saramaeki O. Tanner M. Visakorpi T. Lab. Invest. 82:629-637(2002) [PubMed: 12004003] [Abstract]Cited for: TISSUE SPECIFICITY. [5] \" Phosphorylation of a novel SOCS-box regulates assembly of the HIV-1 Vif-Cul5 complex that promotes APOBEC3G degradation. \" Mehle A. Goncalves J. Santa-Marta M. McPike M. Gabuzda D. Genes Dev. 18:2861-2866(2004) [PubMed: 15574592] [Abstract]Cited for: INTERACTION WITH HIV VIF. [6] \" TMF/ARA160 is a BC-box-containing protein that mediates the degradation of Stat3. \" Perry E. Tsruya R. Levitsky P. Pomp O. Taller M. Weisberg S. Parris W. Kulkarni S. Malovani H. Pawson T. Shpungin S. Nir U. Oncogene 23:8908-8919(2004) [PubMed: 15467733] [Abstract]Cited for: INTERACTION WITH TMF1. [7] \" Respiratory syncytial virus NS1 protein degrades STAT2 by using the Elongin-Cullin E3 ligase. \" Elliott J. Lynch O. T. Suessmuth Y. Qian P. Boyd C. R. Burrows J. F. Buick R. Stevenson N. J. Touzelet O. Gadina M. Power U. F. Johnston J. A. J. Virol. 81:3428-3436(2007) [PubMed: 17251292] [Abstract]Cited for: INTERACTION WITH HRSV PROTEIN NS1. [8] Colinge J. Superti-Furga G. Bennett K. L. Submitted (OCT-2008) to UniProtKBCited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. [9] \" Lysine acetylation targets protein complexes and co-regulates major cellular functions. \" Choudhary C. Kumar C. Gnad F. Nielsen M. L. Rehman M. Walther T. Olsen J. V. Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract]Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-32 MASS SPECTROMETRY. [10] \" Structure of the VHL-ElonginC-ElonginB complex: implications for VHL tumor suppressor function. \" Stebbins C. E. Kaelin W. G. Jr. Pavletich N. P. Science 284:455-461(1999) [PubMed: 10205047] [Abstract]Cited for: X-RAY CRYSTALLOGRAPHY (2. 7 ANGSTROMS) OF 17-112 IN COMPLEX WITH 1-120 OF TCEB2 AND 54-213 OF VHL.