MOUSE ANTI HUMAN CD243

CD243

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Specifically recognises an extracellular conformational epitope of CD243 also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein) a transmembrane protein and member of the ABC transporter (ATP-binding cassette) family which acts as an active efflux pump for a diverse range of lipophillic compounds. CD243 is expressed at low levels in the cell membrane of peripheral blood leucocytes and constitutively expressed on the apical plasma membrane of excretory epithelial cells of the kidney liver brain and small intestine. CD243 mediates resistance to many chemotherapeutic agents used for tumour suppression and is therefore of special interest to oncologists. Clone UIC2 is a strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs. Clone UIC2 can be used in a shift assay to selectively demonstrate the expression and functional activity of CD243 in a target cell (2). Clone UIC2 does not cross-react with mitochondrial pyruvate carboxylase. AbD Recommended Negative Controls: MOUSE IgG2a NEGATIVE CONTROLRecommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGHuCAL Anti Mouse IgG2aRabbit Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMGoat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L)

Genetic variation in ABCB1 may play a role in patients who do not respond to drug treatment. Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast in ulcerative colitis the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin eyes or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases. Ref. 19Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3. A. 1. 201) subfamily. [View classification]Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains. Sequence cautionThe sequence AAM49149. 1 differs from that shown. Reason: Erroneous gene model prediction. [1] \" Ambra1 regulates autophagy and development of the nervous system. \" Maria Fimia G. Stoykova A. Romagnoli A. Giunta L. Di Bartolomeo S. Nardacci R. Corazzari M. Fuoco C. Ucar A. Schwartz P. Gruss P. Piacentini M. Chowdhury K. Cecconi F. Nature 447:1121-1125(2007) [PubMed: 17589504] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Brain. [2] \" Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. \" Nagase T. Kikuno R. Hattori A. Kondo Y. Okumura K. Ohara O. DNA Res. 7:347-355(2000) [PubMed: 11214970] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain. [3] \" Complete sequencing and characterization of 21 243 full-length human cDNAs. \" Ota T. Suzuki Y. Nishikawa T. Otsuki T. Sugiyama T. Irie R. Wakamatsu A. Hayashi K. Sato H. Nagai K. Kimura K. Makita H. Sekine M. Obayashi M. Nishi T. Shibahara T. Tanaka T. Ishii S. Yamamoto J. Saito K. Kawai Y. Isono Y. Nakamura Y. Nagahari K. Murakami K. Yasuda T. Iwayanagi T. Wagatsuma M. Shiratori A. Sudo H. Hosoiri T. Kaku Y. Kodaira H. Kondo H. Sugawara M. Takahashi M. Kanda K. Yokoi T. Furuya T. Kikkawa E. Omura Y. Abe K. Kamihara K. Katsuta N. Sato K. Tanikawa M. Yamazaki M. Ninomiya K. Ishibashi T. Yamashita H. Murakawa K. Fujimori K. Tanai H. Kimata M. Watanabe M. Hiraoka S. Chiba Y. Ishida S. Ono Y. Takiguchi S. Watanabe S. Yosida M. Hotuta T. Kusano J. Kanehori K. Takahashi-Fujii A. Hara H. Tanase T. -O. Nomura Y. Togiya S. Komai F. Hara R. Takeuchi K. Arita M. Imose N. Musashino K. Yuuki H. Oshima A. Sasaki N. Aotsuka S. Yoshikawa Y. Matsunawa H. Ichihara T. Shiohata N. Sano S. Moriya S. Momiyama H. Satoh N. Takami S. Terashima Y. Suzuki O. Nakagawa S. Senoh A. Mizoguchi H. Goto Y. Shimizu F. Wakebe H. Hishigaki H. Watanabe T. Sugiyama A. Takemoto M. Kawakami B. Yamazaki M. Watanabe K. Kumagai A. Itakura S. Fukuzumi Y. Fujimori Y. Komiyama M. Tashiro H. Tanigami A. Fujiwara T. Ono T. Yamada K. Fujii Y. Ozaki K. Hirao M. Ohmori Y. Kawabata A. Hikiji T. Kobatake N. Inagaki H. Ikema Y. Okamoto S. Okitani R. Kawakami T. Noguchi S. Itoh ||?||. Shigeta K. Senba T. Matsumura K. Nakajima Y. Mizuno T. Morinaga M. Sasaki M. Togashi T. Oyama M. Hata H. Watanabe M. Komatsu T. Mizushima-Sugano J. Satoh T. Shirai Y. Takahashi Y. Nakagawa K. Okumura K. Nagase T. Nomura N. Kikuchi H. Masuho Y. Yamashita R. Nakai K. Yada T. Nakamura Y. Ohara O. Isogai T. Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5) NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 464-1298. [4] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Testis. [5] \" Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. \" Daub H. Olsen J. V. Bairlein M. Gnad F. Oppermann F. S. Korner R. Greff Z. Keri G. Stemmann O. Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract]Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-639 MASS SPECTROMETRY. Tissue: Cervix carcinoma. [6] \" A quantitative atlas of mitotic phosphorylation. \" Dephoure N. Zhou C. Villen J. Beausoleil S. A. Bakalarski C. E. Elledge S. J. Gygi S. P. Proc. Natl. Acad. Sci. U. S. A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-639 MASS SPECTROMETRY. Tissue: Cervix carcinoma. [7] \" Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. \" Mayya V. Lundgren D. H. Hwang S. -I. Rezaul K. Wu L. Eng J. K. Rodionov V. Han D. K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract]Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-639 MASS SPECTROMETRY. Tissue: Leukemic T-cell.