SRC3

GWB-479261

Immunogen was a synthetic peptide which represented a portion of human nuclear receptor coactivator 3 encoded within exon 16 (LocusLink ID 8202).

SRC3

1 mg/ml Storage

IP: Use at a concentration of 1 - 4 µ g/ml. WB: Use at an assay dependent dilution. Predicted

Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation it translocates from the cytoplasm to the nucleus. Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which as their names suggest enhance or depress transcriptional activity of the nuclear receptor with which they associate. SRC3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition a polymorphic repeat region is found in the C-terminus of the encoded protein.

Acetyl-CoA + histone = CoA + acetylhistone.

Interacts with CARM1 (By similarity). Present in a complex containing NCOA2 IKKA IKKB IKBKG and the histone acetyltransferase protein CREBBP. Interacts with CASP8AP2 NR3C1 and PCAF. Interacts with ATAD2 and this interaction is enhanced by estradiol.

Widely expressed. High expression in heart skeletal muscle pancreas and placenta. Low expression in brain and very low in lung liver and kidney.

Acetylated by CREBBP. Acetylation occurs in the RID domain and disrupts the interaction with nuclear receptors and regulates its function.

Phosphorylated by IKK complex. Regulated its function.

NCOA3 is frequently amplified or overexpressed in breast and ovarian cancers.

Contains 1 basic helix-loop-helix (bHLH) domain.