Glucagon (ready-to-use)

GWB-FF6038

Full length protein (Pig).

This antibody reacts with human endocrine cells in the pancreas.

Secreted Storage

This antibody has been pretitered and quality controlled to work allon formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However depending upon the fixation conditions and the staining system employed optimal incubation conditions should be determined by the user. Cellular

Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.

GLP-2 stimulates intestinal growth and up-regulates villus height in the small intestine concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis enhancing nutrient assimilation through enhanced gastrointestinal function as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.

Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.

Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1 GLP-2 oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.

Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1 GLP-2 glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas. Pharmaceutical: Available under the names Glucagon (Eli Lilly) and GlucaGen or Glucagon Novo Nordisk (Novo Nordisk). Used to treat severe hypoglycemia in insulin-dependent diabetics.

Belongs to the glucagon family.