Goat anti Apo E

Goat

Apolipoprotein E (Apo E)

Polyclonal Antibodies to Lipoproteins

75mM PBS 75mM Sodium chloride 0. 5mM EDTA pH 7. 2Applications Notes : Can be used for detection of Apo E in plasma and lipoproteins; for immunoassay immunoblot enzyme conjugation biotinylation. Dilution for immunoblot and ELISA: 1:10 000 to 1:80 000 . Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Mediates the binding internalization and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.

Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver brain spleen lung adrenal ovary kidney and muscle.

Glycated in plasma VLDL of normal subjects and of hyperglycemic diabetic patients at a higher level (2-3 fold).

Defects in APOE are a cause of hyperlipoproteinemia type III [MIM:107741]; also known as familial dysbetalipoproteinemia. Individuals with hyperlipoproteinemia type III are clinically characterized by xanthomas yellowish lipid deposits in the palmar crease or less specific on tendons and on elbows. The disorder rarely manifests before the third decade in men. In women it is usually expressed only after the menopause. The vast majority of the patients are homozygous for APOE*2 alleles. More severe cases of hyperlipoproteinemia type III have also been observed in individuals heterozygous for rare APOE variants. The influence of APOE on lipid levels is often suggested to have major implications for the risk of coronary artery disease (CAD). Individuals carrying the common APOE*4 variant are at higher risk of CAD.

Defects in APOE are a cause of sea-blue histiocyte disease [MIM:269600]; also called sea-blue histiocytosis. This disorder is characterized by splenomegaly mild thrombocytopenia and in the bone marrow numerous histiocytes containing cytoplasmic granules which stain bright blue with the usual hematologic stains. The syndrome is the consequence of an inherited metabolic defect analogous to Gaucher disease and other sphingolipidoses.

Belongs to the apolipoprotein A1/A4/E family.