PARP-1 (Cleaved)

GWB-24F761

This antibody only recognizes the p85 cleaved-form of PARP-1. A synthetic peptide corresponding to residues following the cleavage site of human PARP-1 was used as immunogen.

WB: 1:1 000ICC: 1:50FC: 1:50IP: 1:50. Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis PARP is cleaved in vitro by many caspases and in vivo by Caspase-3 (2 3). Existing as a 116 kDa nuclear protein PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa (C-terminal catalytic domain) and 85 kDa (N-terminal DNA-binding domain) (2 4). 1. Yoon S Molloy MJ Wu MP et al C6ORF32 is upregulated during muscle cell differentiation and induces the formation of cellular filopodia Developmental Biology 301 (2007) 70-81[Application: ICC]2. Shangary S Ding K Qiu S et al Reactivation of p53 by a specific MDM2 antagonist (MI-43) leads to p21-mediated cell cycle arrest and selective cell death in colon cancer Mol. Cancer Ther. 7 (2008) 1533 - 1542[Application: WB]3. Doloff JC Waxman DJ Jounaidi Y PARP-1 (Cleaved p85) Hum Gene Ther 19 (2008) 1383-1400[Application: WB]4. Yoon MJ Kim EH Lim JH et al Superoxide anion and proteasomal dysfunction contribute to curcumin-induced paraptosis of malignant breast cancer cells Free Radic Biol Med 48 (2010) 713-726[Application: WB]1. Ikejima M et al. The zinc fingers of human poly(ADP-ribose) polymerase are differentially required for the recognition of DNA breaks and nicks and the consequent enzyme activation. Other structures recognize intact DNA. J. Biol. Chem. 265: 219072. Lazebnik Y. A. et al. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371: 3463. Tewari M et al. Yama/CPP32 beta a mammalian homolog of CED-3 is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81: 8014. Kaufmann S. H. et al. Specific proteolytic cleavage of poly(ADP-ribose) polymerase: An early marker of chemotherapy-induced apoptosis. Cancer Res. 53: 29765. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487