CD4 [Q4120] (PE)

Q4120

CD4-transfected mouse T-cell hybridoma 3DT followed by CD4 + human T-cell CEM cells.

Recognizes the CD4 human cell surface glycoprotein. The antibody blocks the HIV receptor and prevents syncytium formation.

Cell Membrane

0. 01 mg/ml Storage

FACS: Use 10µ l for 10^6 cells. For fluorescence analysis of cells with Fc receptors the use of isotype matched negative control is mandatory. In some systems it may be necessary to incubate the cells (at step 2 before adding monoclonal antibody) in 10-20% normal serum from the second antibody host species in order to decrease non-specific staining with the conjugated second antibody. Monoclonal Anti-Human CD4 may be used for:1. Identification quantification and monitoring of helper/inducer T cells in peripheral blood biological fluids lymphoid organs and other tissues. 2. Analysis of T cell mediated cytotoxicity. 3. Characterization of subtypes of T cell leukemias and lymphomas. 4. Studies of T cells in health and disease. 5. Isolation enrichment or depletion of helper/inducer T lymphocytes. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Cellular

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with and sequesters CD4 in the endoplasmic reticulum.

Primary receptor for HIV-1.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.