Vimentin

IgG1

RV202 reacts exclusively with vimentin. which is expressed in mesenchymal cells and mesenchymal derived tumors e. g. lymphoma. sarcoma and melanoma. RV202 is suitable for immunoblotting. immunocytochemistry. immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:100 ? 1:200 for flow cytometry. and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. and 1:100 ? 1:1000 for immunoblotting applications. Product

Vimentins are class-III intermediate filaments found in various non-epithelial cells especially mesenchymal cells.

Highly expressed in fibroblasts some expression in T- and B-lymphocytes and little or no expression in Burkitt\' s lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Belongs to the intermediate filament family. 1. Ramaekers. F. Huysmans. A. Schaart. G. Moesker. O. and Vooijs. P. (1987). Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp Cell Res 170. 235- 49. 2. Viebahn. C. Lane. E. B. and Ramaekers. F. C. (1988). Keratin and vimentin expression in early organogenesis of the rabbit embryo. Cell Tissue Res 253. 553-62. 3. Pieper. F. R. Schaart. G. Krimpenfort. P. J. Henderik. J. B. Moshage. H. J. van de Kemp. A. Ramaekers. F. C. Berns. A. and Bloemendal. H. (1989). Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells. J Cell Biol 108. 1009-24. 5. Ramaekers. F. van Niekerk. C. Poels. L. Schaafsma. E. Huijsmans. A. Robben. H. Schaart. G. and Vooijs. P. (1990). Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136. 641-55.