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Anti-DNP

Anti-DNP

Manufacturer	GENWAY
Category	Oncology Antibodies Immunoprecipitation Western Blot ELISA Primary Antibodies By Organ Heart & Blood Cancer
Type	Antibody
Specific against	other
Applications	WB, IP, ELISA
Amount	0.1 mg
Item no.	20-732-290015
eClass 6.1	32160702
eClass 9.0	32160702
Available
Genway ID:	GWB-A0691C
Antigen:	DNP
Immunogen:	This monoclonal antibody was produced after repeated immunizations of armenian hamsters with DNP conjugated KLH.
Clone Number:	3E10Family: Epitope TagFraction: IgGPurity
Note:	This product is an IgG fraction antibody purified from ascites or tissue culture supernate by affinity chromatography followed by extensive dialysis against the buffer stated above. Assay by SDS-PAGE resulted in a single protein band. The antibody reacts with DNP but not with carrier protein.
Application Note:	This monoclonal antibody reacts specifically with DNP (2 4-dinitrophenol). The antibody reacts with free DNP DNP conjugated to carriers such as thyroglobulin or BSA and detects the presence of DNP in proteins and nucleic acids. Although not tested this antibody is likely functional in RIA flow cytometry immunohistochemistry and immunoprecipitation. Recommended
Dilution:	This product was assayed against DNP conjugated BSA by ELISA using HRP Goatanti- Armenian Hamster IgG for detection. A working dilution of 1:2 000 to 1:10 000 is suggested for this assay. For immunoblotting dilute the antibody 1:1000 immediately before use. Researchers should determine optimal titers for other applications. Physical State: Liquid (sterile filtered)Hybridoma: Produced by the fusion between armenian hamster splenocytes and mouse myeloma SP2/0 cells using conventional hybridoma technology. Antibody
Concentration:	1. 0 mg/ml (by UV absorbance at 280 nm)
Buffer:	0. 02 M Potassium Phosphate 0. 15 M Sodium Chloride pH 7. 2
Stabilizer:	None
Ship Code:	DRYICE
Function:	Exhibits vasodilator natriuretic and diuretic properties in animal models and human tissues. Acts by stimulating cGMP via the natriuretic peptide receptor A (NPR1). Is a poor agonist of the atrial natriuretic peptide receptor B (NPR2). Is not degradated by neutral endopeptidase (NEP / MME). Binds to atrial natriuretic peptide clearance receptor (NPR3 / NPR-C) which may be responsible of the removal of DNP from the circulation. Increases calcium uptake and induces histamine release from rat peritoneal mast cells. Increases calcium-activated potassium current in gastric antral circular smooth muscle cells by increasing cGMP production and activating inositol trisphosphate receptors (IP3Rs).
Subcellular Location:	Secreted.
Tissue Specificity:	Expressed by the venom gland.
Similarity:	Belongs to the natriuretic peptide family.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

available

Delivery expected until 5/30/2024