Manufacturer |
GENWAY
|
Category |
|
Type |
Antibody |
Specific against |
other |
Clone |
RPA-T4 |
Applications |
FC |
Amount |
25 TESTS |
Item no. |
20-783-310044 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Genway ID: |
GWB-9F83FB |
Specificity: |
CD4NCBI |
Gene ID: |
920 |
Specificity: |
CD4 |
Clone: |
RPA-T4 |
Immunogen: |
Human PHA blasts |
Fusion Partner: |
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. |
Preparation: |
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
Buffer Solution: |
Phosphate buffered saline pH7. 4 |
|
Preservative Stabilisers: |
| 0. 09%Sodium Azide1%Bovine Serum Albumin Reconstitution: Reconstitute in 0. 25 ml disilled waterSuggested |
Flow Cytometry: |
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole bloodSuggested |
Dilution: |
Flow Cytometry - Neat |
Function: |
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. |
Subunit: |
Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. |
Subcellular Location: |
Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with and sequesters CD4 in the endoplasmic reticulum. |
Ptm: |
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. |
Miscellaneous: |
Primary receptor for HIV-1. |
Similarity: |
Contains 3 Ig-like C2-type (immunoglobulin-like) domains. |
Similarity: |
Contains 1 Ig-like V-type (immunoglobulin-like) domain. 1. Zarkesh-Esfahani. H. et al. (2001) High-dose leptin activates human leukocytes via receptor expression on monocytes. 2. Voehringer. D. et al. (2002) Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectin-like receptor G1 (KLRG1). 3. Piatier-Tonneau. D. (1997) CD4 workshop panel report. In Leucocyte Typing VI. White cell differentiation antigens. Edited by Kishimoto. T. . Kikutani. H. . von dem Borne. A. E. G. Kr. . Goyert. A. M. . Mason. D. Y. . Miyasaka. M. . Moretta. L. . Okumura. K. . Shaw. S. . Springer. T. A. . Sugamura. K. . Zola. H. Garland publishing Inc. New York & London. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
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