CD84


Manufacturer	GENWAY
Category	Oncology Antibodies Flow Cytometry Primary Antibodies By Organ Heart & Blood Cancer
Type	Antibody
Specific against	other
Clone	CD84. 1. 21
Applications	FC
Amount	0.025 mg
Item no.	20-783-310243
eClass 6.1	32160702
eClass 9.0	32160702
Available
Genway ID:	GWB-A54186
Specificity:	CD84
Specificity:	CD84
Clone:	CD84. 1. 21
Immunogen:	Mouse pre-B cell line 300. 19 transfected with CD84 cDNA.
Fusion Partner:	Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Preparation:	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:	Phosphate buffered saline
Preservative Stabilisers:		0. 09%Sodium Azide1%Bovine Serum AlbuminSuggested
Flow Cytometry:	Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. Suggested
Dilution:	Flow Cytometry - Neat
Function:	Plays a role as adhesion receptor functioning by homophilic interactions and by clustering. Recruits SH2 domain-containing proteins SH2D1A/SAP. Increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway. May serve as a marker for hematopoietic progenitor cells.
Subunit:	Homodimer; via its extracellular domain. Forms a head to tail dimer with a CD48 molecule from another cell. Interacts with SH2 domain-containing proteins SH2D1A/SAP and SH2D1B/hEAT-2. Interacts with tyrosine-protein phosphatases PTPN6 and PTPN11 via its phosphorylated cytoplasmic domain and this interaction is blocked by SH2D1A.
Subcellular Location:	Cell membrane; Single-pass type I membrane protein.
Tissue Specificity:	Predominantly expressed in hematopoietic tissues such as lymph node spleen and peripheral leukocytes. Expressed in macrophages B-cells monocytes platelets thymocytes T-cells and dendritic cells. Highly expressed in memory T-cells. Developmental Stage: Expression is slightly increased in naive B-cells after the first dividion. By contrast expression on memory B-cells decreased with each successive division.
Domain:	ITSM (immunoreceptor tyrosine-based switch motif) motif is a cytoplasmic motif which may bind SH2D1A.
Ptm:	Phosphorylated by tyrosine-protein kinase LCK on tyrosine residues following ligation induced by agonist monoclonal antibody. The association with SH2D1A/SAP is dependent of tyrosines phosphorylation of its cytoplasmic domain Phosphorylated on Tyr-296 and Tyr-316 following platelet aggregation.
Ptm:	N-glycosylated.
Similarity:	Contains 1 Ig-like C2-type (immunoglobulin-like) domain. 1. Martin. M. et al. (2001) CD84 functions as a homophilic adhesion molecule and enhances IFN-gamma secretion: adhesion is mediated by Ig-like domain 1. 2. Sayos. J. et al. (2001) Cell surface receptors Ly-9 and CD84 recruit the X-linked lymphoproliferative disease gene product SAP.

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Amount: 0.025 mg

available

Delivery expected until 5/30/2024