Antibodies

TRF1

Manufacturer GENWAY
Category Products / Antibody
Type Antibody
Specific against other
Clone BED5
Applications IHC, ELISA
Amount 0.1 mg
ArtNr 20-783-310624
Available
Genway ID:
GWB-75DED1
Specificity:
TRF1NCBI
Gene ID:
7013
Specificity:
TRF1
Clone:
BED5
Immunogen:
Recombinant TRF1
Immunohistology:
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6. 0 is recommended for this purpose Histology: Human Tonsil
Preparation:
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
0. 09%Sodium Azide (NaN3)Suggested
Dilution:
Suggested
Dilution:
Immunohistology - Paraffin - 1/200 - 1/1000
Function:
Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
Subunit:
Homodimer; can contain both isoforms. Found in a complex with POT1; TINF2 and TNKS1. Interacts with ATM TINF2 TNKS1 TNKS2 PINX1 NEK2 and MAPRE1. Component of the shelterin complex (telosome) composed of TERF1 TERF2 TINF2 TERF2IP ACD and POT1.
Subcellular Location:
Nucleus. Spindle. Telomere. Note=Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
Tissue Specificity:
Highly expressed and ubiquitous. Isoform Pin2 predominates.
Induction:
Pin2 expression is tightly regulated during the cell cycle; levels are low in G1 and S phase and increase during G2 phase and mitosis.
Domain:
The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
Ptm:
Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
Ptm:
ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
Similarity:
Contains 1 HTH myb-type DNA-binding domain. 1. Galati. A. et al. (2006) The human telomeric protein TRF1 specifically recognizes nucleosomal binding sites and alters nucleosome structure. 2. de Lange. T. et al (2005) Shelterin: the protein complex that shapes and safeguards human telomeres.
Amount: 0.1 mg
Available: In stock
Listprice: €434.19
Price: €434.19
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