POLY(ADP-RIBOSE) POLYMERASE

GWB-98DF48

142

A6. 4. 12

Spleen cells from immunised BALB/c mice were fused with cells of mouse NSO myeloma cell line.

This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6. 0 is recommended for this purpose.

Western Blotting - 1/1000 - 1/5000 Summary: This protein is a chromatin-associated enzyme poly(ADP-ribosyl)transferase which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation proliferation and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition this enzyme may be the site of mutation in Fanconi anemia and may participate in the pathophysiology of type I diabetes. Summary: This protein is a chromatin-associated enzyme poly(ADP-ribosyl)transferase which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation proliferation and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition this enzyme may be the site of mutation in Fanconi anemia and may participate in the pathophysiology of type I diabetes. 1. Freire. R. et al. (2001) Cleavage of the Bloom\' s syndrome gene product during apoptosis by caspase-3 results in an impaired interaction with topoisomerase IIIalpha. 2. Krohn. A. et al. (1998) Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector.