CD4


Item no.	20-783-74864
Manufacturer	GENWAY
Amount	0.5 mg
Category	Oncology Antibodies Flow Cytometry Primary Antibodies By Organ Heart & Blood Cancer
Type	Antibody
Applications	FC
Clone	YTS177. 9
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-E74090
Similar products	20-783-74864
Available
Genway ID:	GWB-E74090
Specificity:	CD4NCBI
Gene ID:	12504
Specificity:	CD4
Clone:	YTS177. 9
Immunogen:	Mouse spleen cells
Fusion Partner:	Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1. 2. 3 rat myeloma cell line.
Specificity Note:	This product recognises murine CD4 a 55kD cell surface antigen expressed by a subset of T lymphocytes. The antibody is reported to block MHC class II dependant T-cell responses in vitro and in vivo and induces tolerance.
Preparation:	Purified IgG fraction prepared by ammonium sulphate precipitation
Buffer Solution:	Phosphate buffered saline pH7. 4
Preservative Stabilisers:		None presentSuggested
Flow Cytometry:	Use 10ul of the suggested working dilution to label 106 cells in 100ul. Suggested
Dilution:	Flow Cytometry - 1/50 - 1/200
Function:	Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
Subunit:	Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins.
Subcellular Location:	Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with and sequesters CD4 in the endoplasmic reticulum.
Ptm:	Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
Miscellaneous:	Primary receptor for HIV-1.
Similarity:	Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Similarity:	Contains 1 Ig-like V-type (immunoglobulin-like) domain. 1. Qin. S. et al. (1990) Induction of tolerance in peripheral T-cells with monoclonal antibodies. 2. Cobbold. S. P. et al. (1990) The induction of skin graft tolerance in MHC-mismatched or primed recipients: primed T-cells can be tolerised in the periphery with CD4 and CD8 antibodies. 3. Wise. M. P. et al. (1998) Cutting Edge: Linked suppression of skin graft rejection can operate through indirect recognition.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.5 mg

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Delivery expected until 11/27/2025

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