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AMPK1/AMPK2 (Phospho-Ser485/ Ser491) Antibody Blocking Peptide

AMPK1/AMPK2 (Phospho-Ser485/ Ser491) Antibody Blocking Peptide

Catalytic
Item no.	06-785-213147
Manufacturer	GENWAY
Amount	0.1 mg
Category	Oncology Metabolism By Cell Type Hematology Lymphoma Peptides & Proteins Cell Biology Obesity Cell Status Autophagy Peptides
Type	Peptides
Specific against	other
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	GWB-C21E16
Similar products	06-785-213147
Available
Genway ID:	GWB-C21E16
Form of Peptide:	Peptide in 10 mM phosphate buffered saline (without Mg2+ and Ca2+) pH 7. 4 150mM NaCl 0. 1 mM EDTA 1 mg/ml BSA 5% DMF and 5% glycerol. Peptide Information: The synthesized phosphopeptide was derived from human AMPK1/AMPK2around the phosphorylation site of serine 485/491 (S-G- SP-V-S). Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and mass spectrometry.
Specificity:	The peptide specifically blocks the signal of AMPK1/AMPK2 (Phospho-Ser485/Ser491) antibody (#11174) completely in Western blotting and IHC.
Applications:	For
Western Blotting:	add 10 ?l of antibody and 10 ?l of blocking peptide to 10 ml of antibody dilution buffer and incubate at 4? over night or at room temperature for 2 hours before allowing to react with the blot.
Function:	Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5\' -AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Activity:	ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium.
Enzyme Regulation:	Binding of AMP results in allosteric activation inducing phosphorylation on Thr-174 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions without detectable changes in the AMP/ATP ratio.
Subunit:	Heterotrimer of an alpha catalytic subunit a beta and a gamma non-catalytic subunits. Interacts with FNIP1 and FNIP2.
Similarity:	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Similarity:	Contains 1 protein kinase domain.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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Delivery expected until 10/30/2025

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