RRM1 (ribonucleotide reductase M1 polypeptide)

GWB-625B39

1:312500

Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. RRM1 is one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. Its gene is located in the imprinted gene domain of 11p15. 5 an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome Wilms tumor rhabdomyosarcoma adrenocrotical carcinoma and lung ovarian and breast cancer. Its gene may play a role in malignancies and disease that involve this region. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests. This gene encodes one of two non-identical subunits which constitute ribonucleoside-diphosphate reductase an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15. 5 an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome Wilms tumor rhabdomyosarcoma adrenocrotical carcinoma and lung ovarian and breast cancer. This gene may play a role in malignancies and disease that involve this region. This gene is oriented in a head-to-tail configuration with the stromal interaction molecule 1 gene (STIM1) with the 3\' end of STIM1 situated 1. 6 kb from the 5\' end of this gene.

2\' -deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Genetic information processing; DNA replication.

Cytoplasm.

Two distinct regulatory sites have been defined: the specificity site which controls substrate specificity and the activity site which regulates overall catalytic activity. A substrate-binding catalytic site located on M1 is formed only in the presence of the second subunit M2.

Belongs to the ribonucleoside diphosphate reductase large chain family.