MeCP2 Antibody

Synthetic Peptide

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Polyclonal

4204

Affinity Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-MeCP2(S423) Antibody at 1:2000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: A431 whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: Mouse brain lysate


Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 52 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

WB

AP2545D staining MeCP2 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/500) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

1:500

IHC-P

Q95LG8

52441

MACFA