RELA Antibody (N-term)

Synthetic Peptide

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL(MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).

Polyclonal

5970

Purified Rabbit Polyclonal Antibody (Pab)

Confocal immunofluorescent analysis of RELA Antibody (N-term)(Cat#AP6826a) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

1:10~50

WB

RELA Antibody (N-term) (Cat. #AP6826a) western blot analysis in Hela, MCF-7, Raji, Ramos cell line lysates (35ug/lane).This demonstrates the RELA antibody detected the RELA protein (arrow).

1:1000

IHC-P

RELA Antibody (N-term) (Cat. #AP6826a) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

1:10~50

C, X

60219

HUMAN

NP_001138610.1; NP_001230913.1; NP_068810.3