Comparison

GALT Antibody (C-term)

Item no. ABC-AP6880b
Manufacturer Abcepta
Amount 400 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications WB, FC, IHC-P
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products GALT, Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase
Available
Primary Accession
P07902
Antigen Type
Synthetic Peptide
Application
FC, IHC-P, WB
Bio Background
Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet.
Bio References
Leslie, N.D., et.al., Genomics 14 (2), 474-480 (1992)
Clonality
Polyclonal
Clone/Animal Names
RB20969
Gene ID
2592
Gene Name
GALT
Subtitle
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H
Legend image 1
Anti-GALT Antibody (C-term) at 1:1000 dilution + HepG2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 43 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1
WB
Dilution image 1
1:1000
Legend image 2
Western blot analysis of GALT Antibody (C-term) (Cat. #AP6880b) in MDA-MB231 cell line lysates (35ug/lane). GALT (arrow) was detected using the purified Pab.
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with GALT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3
IHC-P
Dilution image 3
1:50~100
Legend image 4
Flow cytometric analysis of MDA-MB468 cells using GALT Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4
FC
Dilution image 4
1:10~50
Isotype
Rabbit Ig
Calculated MW
43363
Antigen Region
344-373
Antigen Source
HUMAN
Target/Specificity
This GALT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344-373 amino acids from the C-terminal region of human GALT.
NCBI Accession
NP_000146.2; NP_001245261.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
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