FGR Antibody (N-term)

Synthetic Peptide

FGR is a member of the Src family of protein tyrosine kinases (PTKs). This protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. It localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this protein.

Polyclonal

2268

Purified Rabbit Polyclonal Antibody (Pab)

Confocal immunofluorescent analysis of FGR Antibody (N-term)(Cat#AP7707a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

1:10~50

WB

Anti-FGR Antibody (N-term) at 1:2000 dilution + Raji whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 59 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

WB

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

1:50~100

59479

HUMAN

NP_001036194.1; NP_001036212.1; NP_005239.1