Comparison

GAPDHS Antibody (Center)

Item no. ABC-AP8610c
Manufacturer Abcepta
Amount 400 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applications WB, FC, IHC-P
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products GAPDH-2, GAPDHS, Glyceraldehyde-3-phosphate dehydrogenase, testis-specific, GAPD2, GAPDS, GAPDH2, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2, Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
Available
Primary Accession
O14556
Antigen Type
Synthetic Peptide
Application
FC, IHC-P, WB
Bio Background
GAPDHS is a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1, 3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility.
Bio References
Welch, J.E., et.al., J. Androl. 21 (2), 328-338 (2000) Goodwin, L.O., et.al., Mol. Hum. Reprod. 6 (2), 127-136 (2000) Benham, F.J. et.al., Genomics 5 (2), 209-214 (1989)
Clonality
Polyclonal
Clone/Animal Names
RB16542
Gene ID
26330
Gene Name
GAPDHS
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H
Legend image 1
Western blot analysis of GAPDHS Antibody (Center) (Cat. #AP8610c) in NCI-H460 cell line lysates (35ug/lane). GAPDHS (arrow) was detected using the purified Pab.
Type image 1
WB
Dilution image 1
1:1000
Legend image 2
GAPDHS Antibody (Center) (Cat. #AP8610c) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GAPDHS Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Type image 2
IHC-P
Dilution image 2
1:50~100
Legend image 3
GAPDHS Antibody (Center) (Cat. #AP8610c) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3
FC
Dilution image 3
1:10~50
Other Accession
Q4R3T1
Predicted
Pr
Isotype
Rabbit Ig
Calculated MW
44501
Antigen Region
104-134
Antigen Source
HUMAN
Target/Specificity
This GAPDHS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-134 amino acids from the Central region of human GAPDHS.
NCBI Accession
NP_055179.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
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