Comparison

IGH Antibody (C-Term)

Item no. ABC-AP8783b
Manufacturer Abcepta
Amount 400 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications WB, FC, IHC-P
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products IGHA1, Ig alpha-1 chain C region
Available
Primary Accession
P01876
Antigen Type
Synthetic Peptide
Application
IHC-P, FC, WB
Bio References
Strausberg, R.L., et.al., Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
Clonality
Polyclonal
Clone/Animal Names
RB23640
Gene Name
IGHA1 {ECO:0000303|PubMed:11340299, ECO:0000303|Ref.12}
Subtitle
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H
Legend image 1
Western blot analysis of IGH Antibody (C-Term) (Cat. #AP8783b) in HL-60, A2058 cell line lysates (35ug/lane). IGH (arrow) was detected using the purified Pab.
Type image 1
WB
Dilution image 1
1:1000
Legend image 2
Anti-IGH Antibody (C-Term) at 1:1000 dilution + A2058 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 38 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
Formalin-fixed and paraffin-embedded human skin reacted with IGH Antibody (C-Term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3
IHC-P
Dilution image 3
1:50~100
Legend image 4
IGH Antibody (C-Term) (Cat. #AP8783b) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4
FC
Dilution image 4
1:10~50
Other Accession
P01877
Isotype
Rabbit Ig
Calculated MW
37655
Antigen Region
290-320
Antigen Source
HUMAN
Target/Specificity
This IGH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-320 amino acids from the C-terminal region of human IGH.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
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