Comparison

PIN1 Antibody (Center)

Item no. ABC-AP8959C
Manufacturer Abcepta
Amount 400 ul
Category
Type Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications WB, FC, IF, IHC-P
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products PIN1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1, Rotamase Pin1, PPIase Pin1
Available
Primary Accession
Q13526
Antigen Type
Synthetic Peptide
Application
IF, WB, FC, IHC-P
Bio Background
PIN1 is an essential nuclear peptidylprolyl cis-trans isomerase (PPIase; EC 5.2.1.8) involved in regulation of mitosis.
Bio References
Lu, K.P., et.al., Nature 380 (6574), 544-547 (1996) Campbell, H.D., et.al., Genomics 44 (2), 157-162 (1997)
Clonality
Polyclonal
Clone/Animal Names
RB22881
Gene ID
5300
Gene Name
PIN1
Subtitle
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H
Legend image 1
Confocal immunofluorescent analysis of PIN1 Antibody (Center) (Cat. #AP8959c) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Type image 1
IF
Dilution image 1
1:10~50
Legend image 2
Western blot analysis of PIN1 Antibody (Center) (Cat. #AP8959c) in HL-60 cell line lysates (35ug/lane). PIN1 (arrow) was detected using the purified Pab.
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
Western blot analysis of PIN1 (arrow) using rabbit polyclonal PIN1 Antibody (Center) (Cat. #AP8959c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIN1 gene.
Type image 3
WB
Dilution image 3
1:1000
Legend image 4
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PIN1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 4
IHC-P
Dilution image 4
1:50~100
Legend image 5
PIN1 Antibody (Center) (Cat.#AP8959c) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 5
FC
Dilution image 5
1:10~50
Other Accession
Q4R383
Predicted
Pr
Isotype
Rabbit Ig
Calculated MW
18243
Antigen Region
30-56
Antigen Source
HUMAN
Target/Specificity
This PIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-56 amino acids from the Central region of human PIN1.
NCBI Accession
NP_006212.1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 400 ul
Available: In stock
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