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NKX3-1 Antibody (Center)

NKX3-1 Antibody (Center)

Item no.	ABC-AP8996C
Manufacturer	Abcepta
Amount	400 ul
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	NKX3-1, NKX3A, Homeobox protein NK-3 homolog A, NKX31, Homeobox protein Nkx-31
Available
Primary Accession	Q99801
Antigen Type	Synthetic Peptide
Application	FC, IHC-P, WB
Bio Background	NKX3-1 is a transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. It play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. It Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
Bio References	Voeller, H.J., et.al., Cancer Res. 57 (20), 4455-4459 (1997)
Clonality	Polyclonal
Clone/Animal Names	RB23610
Gene ID	4824
Gene Name	NKX3-1
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Anti-NKX3-1 Antibody (Center) at 1:8000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:8000
Legend image 2	Western blot analysis of NKX3-1 Antibody (Center) (Cat. #AP8996c) in MCF-7 cell line lysates (35ug/lane). NKX3-1 (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with NKX3-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:50~100
Legend image 4	NKX3-1 Antibody (Center) (Cat. #AP8996c) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	P97436
Predicted	M
Isotype	Rabbit Ig
Calculated MW	26350
Antigen Region	3-1
Antigen Source	HUMAN
Target/Specificity	This NKX3-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-145 amino acids from the Central region of human NKX3-1.
NCBI Accession	NP_001243268.1; NP_006158.2

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 400 ul

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Delivery expected until 11/6/2025

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