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Anti-Mouse NK1.1 (PK136) In Vivo Antibody - Ultra-Low Endotoxin

Anti-Mouse NK1.1 (PK136) In Vivo Antibody - Ultra-Low Endotoxin

Item no.	ICH1128UL-100MG
Manufacturer	IchorBio
Amount	100 mg
Quantity options	100 mg 25 mg 50 mg 5 mg
Category	Antibodies In Vivo Antibodies Flow Cytometry Western Blot Blocking
Type	Antibody
Applications	WB, FC, IP, IHC, Blocking
Clone	PK136
Specific against	Mouse (Murine, Mus musculus)
Host	Mouse
Isotype	IgG2a Kappa
Purity	>98% by SDS-PAGE and HPLC
Citations	Yang et al. Aconite aqueous extract inhibits the growth of hepatocellular carcinoma through CCL2-dependent enhancement of natural killer cell infiltration. Journal of Integrative Medicine
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Natural Killer Cells, KR-P1C, NKR-P1B, Ly-55, CD161b, CD161c
Shipping condition	Cool pack
Available
Specificity	Anti-NK1.1 In Vivo Antibody - Ultra Low Endotoxin (PK136) recognizes an epitope on Mouse NK1.1
Manufacturer - Applications	Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, CyTOF, IHC (Frozen)
Manufacturer - Category	Ultra-Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype	Mouse IgG2a Kappa
Manufacturer - Targets	NK1.1
Shipping Temperature	Blue ice
Storage Conditions	anti-NK1.1 In Vivo Antibody - Ultra Low Endotoxin (PK136) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Aggregation	Aggregation level ≤ 1%
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution	Mouse NK cells. Endogenous and interferon induced NK cells. Not present on cytotoxic T-cells and Con A stimulated blast cells. May be present on a certain subset of cytotoxic T-cells strains A, AKR, BALB/C, BALB.H-2b, CBA, C3H, DBA/2, 124 were negative using PK136.
Application Notes	Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. Each investigator should determine their own optimal working dilution for specific applications.
Background	NK-1.1 surface antigen, also known as CD161b/CD161c, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.
Concentration	≥ 2.0 mg/ml
Endotoxin	≤ 0.5 EU/mg as determined by the LAL method
Manufacturer - Specificity	Anti-NK1.1 In Vivo Antibody - Ultra Low Endotoxin (PK136) recognizes an epitope on Mouse NK1.1
Purification method	This monoclonal antibody was purified using Protein A
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen	NK-1+ cells from mouse spleen and bone marrow
IMPACT Pathogen Test	We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp. Mycoplasma pulmonis Sendai virus Mouse hepatitis virus Pneumonia virus of mice Minute virus of mice Mouse parvovirus (MPV1-5) Theiler's murine encephalomyelitis virus Murine norovirus Reovirus 3 Mouse rotavirus Ectromelia virus Lymphocytic choriomeningitis virus Polyoma virus Lactate dehydrogenase-elevating virus Mouse adenovirus (MAD1, MAD2) Mouse cytomegalovirus K virus Mouse thymic virus Hantaan virus Corynebacterium bovis Corynebacterium spp. (HAC2)
Related Antibodies	Anti-NK1.1 In Vivo Antibody - Low Endotoxin [PK136] (ICH1128)
Isotype Control	Mouse IgG2a In Vivo Isotype Control - Ultra Low Endotoxin [C1.18.4] (ICH2245UL)
Buffer	ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 mg

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Delivery expected until 7/17/2025