Comparison

Anti-Mouse TCR V Gamma 2 (UC3-10A6) In Vivo Antibody - Low Endotoxin European Partner

Item no. ICH1140-100MG
Manufacturer IchorBio
Amount 100 mg
Quantity options 100 mg 1 mg 25 mg 50 mg 5 mg
Category
Type Antibody
Applications FC, IP, IV
Clone UC3-10A6
Specific against Mouse (Murine, Mus musculus)
Host Hamster - Armenian
Isotype IgG
Purity >95% by SDS-PAGE and HPLC
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias V gamma 2
Shipping condition Cool pack
Available
Manufacturer - Applications
Flow Cytometry, Immunoprecipitation, In vivo Depletion
Manufacturer - Category
Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype
Armenian Hamster IgG
Manufacturer - Targets
TCR V gamma 2
Shipping Temperature
Blue ice
Storage Conditions
anti-TCR V gamma 2 In Vivo Antibody - Low Endotoxin (UC3-10A6) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Aggregation
Aggregation level ≤ 5%
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
V gamma 2 T-Cell Receptor (TCR)-bearing T lymphocytes make up a significant proportion of gamma delta TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung.
Application Notes
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TCR. A suggested method would be to stain cells expressing TCR with Anti-Mouse TCR at ≤1.0 µg per 1.0 X 106 cells in a 100 µl total staining volume, followed by Goat Goat Anti-Armenian Hamster IgG (H&L)-R-phycoerythrin
Each investigator should determine their own optimal working dilution for specific applications.
Background
The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an alpha and beta chain in 95% of T cells, while 5% of T cells have TCRs consisting of gamma and delta chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.
Concentration
1.0 - 5.0 mg/ml
Endotoxin
<1.0 EU/mg as determined by the LAL method
Manufacturer - Specificity
This antibody reacts with Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes.
Purification method
This monoclonal antibody was purified using Protein A
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Related Antibodies
Isotype Control
Armenian Hamster IgG Isotype Control for In Vivo - Low Endotoxin [PIP] (ICH2251)
Buffer
ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 mg
Available: In stock
available

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