Manufacturer |
IchorBio
|
Category |
|
Type |
Antibody |
Specific against |
Mouse |
Clone |
RB6-8C5 |
Isotype |
IgG2b |
Applications |
WB, FC, IP, IHC, ICC, FA |
Amount |
5mg |
Host |
Rat |
Item no. |
ICH1131UL-5MG |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Manufacturers Product Category |
Ultra-Low Endotoxin, anti-mouse |
Target Information |
Ly-6G/Ly-6C |
Isotype Information |
Rat IgG2b |
Purification method |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
Antigen Distribution |
The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells. |
Background |
Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C. |
Immunogen |
Raised against granulocytes of mouse origin |
Concentration |
0.5 mg/ml |
Formulation |
No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl. |
Endotoxin |
≤ 0.5 EU/mg as determined by the LAL method |
Aggregation |
Aggregation level ≤ 1% |
IMPACT Pathogen Test |
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: - Mycoplasma spp - Mycoplasma pulmonis - Pneumonia virus of mice - Kilham's rat virus - Toolan's H1 virus - Rat parvovirus - Lymphocytic choriomeningitis virus - Rat cytomegalovirus - Sendai virus - Rat coronavirus - Sialodacryoadenitis virus - Rat minute virus - Seoul virus - Mouse adenovirus - Reovirus 3 - Rat theilovirus |
Storage |
anti-Ly-6G/Ly-6C In Vivo Antibody - Ultra Low Endotoxin (RB6-8C5) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
Application Notes |
Each investigator should determine their own optimal working dilution for specific applications. |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.