Comparison

Epidermal Growth Factor

Epidermal growth factor (EGF) is a protein that stimulates the growth of various epidermal and epithelial tissues1. It consists of 54 amino acids (6.3 kDa), which are linked by 3 intramolecular disulfide bonds2. It occurs increasingly in the initiation of mitosis. For research purposes it can be used both in vitro and in vivo. In some fibroblasts it also stimulates the described growth in cell culture1.

 

epidermal growth factor (EGF)

Fig. 1: Amino acid sequence of the epidermal growth factor (EGF).

Epidermal growth factor receptor

EGF is one of the major ligands of the epidermal growth factor receptor family (EGFR). The receptor belongs to the class of transmembrane glycoproteins and consists of an extracellular receptor domain, a transmembrane region and an intracellular domain which functions as a tyrosine kinase3. When EGF binds to EGFR, autophosphorylation of the receptor tyrosine kinase takes place, which leads to the activation of certain signal transduction pathways. Among other pathways, cell proliferation and cell differentiation are regulated by this type of signal transduction 4.

 

epidermal growth factor receptor (EGFR)

Fig. 2: EGFR consists of an extracellular receptor domain, a transmembrane region and an intracellular domain, which acts as a tyrosine kinase.

 

Research areas

EGFR plays a special role in the study of cancer, as it is overexpressed in a variety of tumor cells. Overexpression of EGFR has been found particularly in solid tumors, e.g. Breast cancer, head and neck cancer, non-small cell lung cancer (NSCLC), kidney cancer, ovarian cancer and colon cancer 5. The activation of the receptor is also associated with resistance to chemotherapy and radiation. The described properties of EGFR make the receptor an important therapeutic target in the treatment of cancer, since interactions with EGFR may lead to an interruption of signal transduction 6.

Particularly inhibitors of EGFR were monoclonal antibodies directed against the extracellular receptor domain and low molecular weight compounds7. Both monoclonal antibodies and low molecular weight compounds can cause cell proliferation to be disrupted and apoptosis facilitated. Consequently, the cytotoxic as well as cytostatic properties can be attributed to the substances mentioned 7.

 

epidermal growth factor (EGF) - pathway

Fig. No. 3: The figure shows a detailed overview of the EGFR signaling pathway.

 

 

 

Products

Antibody

RayBiotech

Name

Typ

Specific against

Host

ArtNr.

Menge

Rabbit Anti-­EGF Receptor

Antibody

Rabbit

other

127-10497

100ul

Mouse Anti-­Human EGF

Antibody

Mouse

Hu

128-10066-1

500 ug

Mouse Anti-­Human EGF

Antibody

Mouse

Hu

128-10066-2

1mg

Goat Anti-­Mouse EGF

Antibody

Goat

Mu

DS-PB-00691

0.1 mg

Goat Anti-­Mouse EGF [+Biotin]

Antibody

Goat

Mu

DS-PB-00692

50 ug

Rabbit Anti-­Human EGF

Antibody

Rabbit

Hu

DS-PB-00693

0.1 mg

Rabbit Anti-­Human EGF [+Biotin]

Antibody

Rabbit

Hu

DS-PB-00694

50 ug

Mouse Anti-­EGFR

Antibody

Mouse

other

MD-02-0003

100 ug

Mouse Anti-­EGFR

Antibody

Mouse

other

MD-02-0004

100 ug

Mouse Anti-­Human EGFR

Antibody

Mouse

Hu

MD-02-0005

100 ug

 

Cloud-Clone

Name

Typ

Specific against

Host

ArtNr.

Menge

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Hu

Mouse

MAA560Hu22_100ug

100ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Po

Mouse

MAA560Po21_100ug

100ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Ra

Mouse

MAA560Ra21_100ug

100ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Hu

Mouse

MAA560Hu22_20ug

20ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Po

Mouse

MAA560Po21_20ug

20ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Ra

Mouse

MAA560Ra21_20ug

20ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Po

Mouse

MAA560Po21_200ug

200ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Ra

Mouse

MAA560Ra21_200ug

200ug

Monoclonal Antibody to Epidermal Growth Factor (EGF)

Antibody Monoclonal

Hu

Mouse

MAA560Hu22_200ug

200ug

Monoclonal Antibody to Epidermal Growth Factor Receptor 2 (EGFR2)

Antibody Monoclonal

Hu

Mouse

MAB867Hu22_200ug

200ug

Monoclonal Antibody to Epidermal Growth Factor Receptor (EGFR)

Antibody Monoclonal

Hu

Mouse

MAA757Hu22_200ug

200ug

 

Sino Biological Inc.

Name

Typ

Specific against

Host

ArtNr.

Menge

Epidermal Growth Factor/­EGF Antibody, Rabbit PAb, Antigen Affinity Purified

Antibody

other

 

101619-T32-50

50 ug

Epidermal Growth Factor/­EGF Antibody, Rabbit PAb, Antigen Affinity Purified

Antibody

other

 

101619-T32-100

100 ug

Epidermal Growth Factor/­EGF Antibody, Rabbit PAb, Antigen Affinity Purified

Antibody

other

 

101619-T32-200

200 ug

Epidermal Growth Factor /­ EGF Antibody, Mouse MAb

Antibody

other

 

80446-MM02-50

50 ug

Epidermal Growth Factor /­ EGF Antibody, Mouse MAb

Antibody

other

 

80446-MM02-100

100 ug

Epidermal Growth Factor /­ EGF Antibody, Mouse MAb

Antibody

other

 

80446-MM02-200

200 ug

EGFR /­ HER1 Antibody, Rabbit MAb

Antibody

other

 

10001-RE11-50

50 ug

EGFR /­ HER1 Antibody, Rabbit MAb

Antibody

other

 

10001-RE11-100

100 ug

EGFR /­ HER1 Antibody, Rabbit MAb

Antibody

other

 

10001-RE11-1

1 mg

EGFR /­ HER1 /­ ErbB1 Antibody (APC), Mouse MAb

Antibody

other

 

10001-MM08-A-25

25 Tests

EGFR /­ HER1 /­ ErbB1 Antibody (APC), Rabbit MAb

Antibody

other

 

10001-RE01-A-25

25 Tests

EGFR /­ HER1 /­ ErbB1 Antibody (PE), Mouse MAb

Antibody

other

 

10001-MM08-P-25

25 Tests

EGFR /­ HER1 /­ ErbB1 Antibody (PE), Rabbit MAb

Antibody

other

 

10001-RE01-P-25

25 Tests

EGFR /­ HER1 /­ ErbB1 Antibody (FITC), Mouse MAb

Antibody

other

 

10001-MM08-F-25

25 Tests

EGFR /­ HER1 /­ ErbB1 Antibody (FITC), Rabbit MAb

Antibody

other

 

10001-RE01-F-25

25 Tests

EGFR /­ HER1 /­ ErbB1 Antibody, Rabbit MAb

Antibody

other

 

10001-R021-1

1 mg

 

ELISA-Kit

RayBiotech Inc.

Name

Typ

Host

ArtNr.

Menge

Human EGF ELISA

Elisa-Kit

E.coli

ELH-EGF-1

1 x 96-Well Strip Microplate Kit

Human EGF ELISA

Elisa-Kit

E.coli

ELH-EGF-2

2 x 96-Well Strip Microplate Kit

Human EGF ELISA

Elisa-Kit

E.coli

ELH-EGF-5

5 x 96-Well Strip Microplate Kit

Mouse EGF ELISA

Elisa-Kit

E.coli

ELM-EGF-1

1 x 96-Well Strip Microplate Kit

Mouse EGF ELISA

Elisa-Kit

E.coli

ELM-EGF-2

2 x 96-Well Strip Microplate Kit

Mouse EGF ELISA

Elisa-Kit

E.coli

ELM-EGF-5

5 x 96-Well Strip Microplate Kit

Rhesus Monkey EGF ELISA

Elisa-Kit

 

ELK-EGF-1

1 x 96-Well Strip Microplate Kit

Rhesus Monkey EGF ELISA

Elisa-Kit

 

ELK-EGF-2

2 x 96-Well Strip Microplate Kit

Rhesus Monkey EGF ELISA

Elisa-Kit

 

ELK-EGF-5

5 x 96-Well Strip Microplate Kit

Custom Human EGF SpeedELISA

Elisa-Kit

 

ELHS-EGF-1

1 x 96-Well Strip Microplate Kit

Custom Human EGF SpeedELISA

Elisa-Kit

 

ELHS-EGF-2

2 x 96-Well Strip Microplate Kit

Custom Human EGF SpeedELISA

Elisa-Kit

 

ELHS-EGF-5

5 x 96-Well Strip Microplate Kit

Rat EGF ELISA

Elisa-Kit

E.coli

ELR-EGF-1

1 x 96-Well Strip Microplate Kit

 

Sino Biological Inc.

Name

Typ

Specific against

ArtNr.

Menge

Human EGFR /­ HER1 /­ ErbB1 ELISA Kit

Elisa-Kit

Hu

KIT10001

1 Kit (96 Tests)

Human EGFR /­ HER1 /­ ErbB1 ELISA Kit

Elisa-Kit

Hu

KIT10001-1

96 Tests

 

 

Cloud-Clone Corp.

Name

Typ

Specific against

ArtNr.

Menge

Mini Samples ELISA Kit for Epidermal Growth Factor (EGF)

Elisa-Kit

other

MEA560Mu

96T

ELISA Kit for Epidermal Growth Factor (EGF)

Elisa-Kit

Hu

SEA560Hu

96T

ELISA Kit for Epidermal Growth Factor (EGF)

Elisa-Kit

Mu

SEA560Mu

96T

ELISA Kit for Epidermal Growth Factor (EGF)

Elisa-Kit

Po

SEA560Po

96T

ELISA Kit for Epidermal Growth Factor (EGF)

Elisa-Kit

Ra

SEA560Ra

96T

ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Elisa-Kit

Ra

SEB286Ra

96T

ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Elisa-Kit

Hu

SEB286Hu-5

5x96T

ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Elisa-Kit

Mu

SEB286Mu-5

5x96T

ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Elisa-Kit

Ra

SEB286Ra-5

5x96T

ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Elisa-Kit

Hu

SEB286Hu-10

10x96T

ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Elisa-Kit

Mu

SEB286Mu-10

10x96T

ELISA Kit for Milk Fat Globule EGF Factor 8 (MFGE8)

Elisa-Kit

Ra

SEB286Ra-10

10x96T

 

Proteins

RayBiotech Inc.

Name

Typ

Specific against

Host

ArtNr.

Menge

Human EGF, HEK293 Lysate

Proteins

other

 

230-10053-100

100 ug

Human EGF, HEK293 Transfected Cell Culture Supernatant

Proteins

other

 

230-20031-200

200 ul

Native Rat EGF

Proteins

Ra

 

DS-01-0091

10 ug

Human EGF

Proteins

Hu

 

213-10068-2

500 ug

EGF, Animal Free, Recombinant Mouse

Proteins

Mu

 

213-10069-AF-1

100 ug

EGF, Animal Free, Recombinant Mouse

Proteins

Mu

 

213-10069-AF-2

500 ug

Native Mouse EGF

Proteins

Mu

 

228-10361-2

50 ug

Native Rat EGF

Proteins

Ra

 

228-10363-2

5 ug

Native Rat EGF

Proteins

Ra

 

228-10363-3

10 ug

Neuregulin-­1/­Heregulin Alpha (EGF Domain), Human Recombinant

Proteins

Hu

 

228-20231-2

20 ug

Neuregulin-­1/­Heregulin Alpha (EGF Domain), Human Recombinant

Proteins

Hu

 

228-20231-3

1 mg

Proheparin-­Binding EGF-­like Growth Factor, Rat Recombinant

Proteins

Ra

 

228-20114-2

50 ug

Proheparin-­Binding EGF-­like Growth Factor, Rat Recombinant

Proteins

Ra

 

228-20114-3

1 mg

Recombinant Rat EGF

Proteins

Ra

 

229-20329

100 ug

Recombinant Human EGF

Proteins

Hu

 

229-20136

500 ug

Epidermal Growth Factor (EGF), Human recombinant

Proteins

Hu

 

268-10048-1

100 ug

Epidermal Growth Factor (EGF), Human recombinant

Proteins

Hu

 

268-10048-2

500 ug

Epidermal Growth Factor (EGF), Human recombinant

Proteins

Hu

 

268-10048-3

1 mg

Epidermal Growth Factor (EGF), Human recombinant

Proteins

Hu

 

268-10048-4

5 mg

EGF, Mouse recombinant

Proteins

Mu

 

268-10049-1

100 ug

EGF, Mouse recombinant

Proteins

Mu

 

268-10049-2

500 ug

EGF, Mouse recombinant

Proteins

Mu

 

268-10049-3

1 mg

EGF, Mouse recombinant

Proteins

Mu

 

268-10049-4

5 mg

EGF, Rat recombinant

Proteins

Ra

 

268-10050-1

20 ug

EGF, Rat recombinant

Proteins

Ra

 

268-10050-2

100 ug

 

Sino Biological Inc.

Name

Typ

Specific against

Host

ArtNr.

Menge

Human EGF /­ Epidermal Growth Factor Protein

Proteins

Hu

E.coli

10605-HNAE-1

1 mg

Human EGF /­ Epidermal Growth Factor Protein

Proteins

Hu

E.coli

10605-HNAE-500

500 ug

Human EGF /­ Epidermal Growth Factor Protein

Proteins

Hu

E.coli

10605-HNAE-100

100 ug

Mouse EGF /­ Epidermal Growth Factor Protein

Proteins

Mu

Human

50482-MNCH-1

1 mg

Mouse EGF /­ Epidermal Growth Factor Protein

Proteins

Mu

Human

50482-MNCH-500

500 ug

Mouse EGF /­ Epidermal Growth Factor Protein

Proteins

Mu

Human

50482-MNCH-100

100 ug

Human EGF /­ Epidermal Growth Factor Protein (Fc Tag)

Proteins

Hu

Human

10605-H01H-1

1 mg

Human EGF /­ Epidermal Growth Factor Protein (Fc Tag)

Proteins

Hu

Human

10605-H01H-500

500 ug

Human EGF /­ Epidermal Growth Factor Protein (Fc Tag)

Proteins

Hu

Human

10605-H01H-100

100 ug

Human NRG1-­beta 1 Protein (EGF Domain, Fc Tag)

Proteins

Hu

Human

11609-H01H-100

100 ug

Human NRG1-­beta 1 Protein (EGF Domain, Fc Tag)

Proteins

Hu

Human

11609-H01H-50

50 ug

Human NRG1-­beta 1 Protein (EGF Domain, Fc Tag)

Proteins

Hu

Human

11609-H01H-5

5 ug

Human NRG1-­alpha Protein (EGF Domain, Fc Tag)

Proteins

Hu

Human

13499-H01H-50

50 ug

Human NRG1-­alpha Protein (EGF Domain, Fc Tag)

Proteins

Hu

Human

13499-H01H-100

100 ug

Canine NRG1-­alpha Protein (EGF Domain, Fc Tag)

Proteins

Ca

Human

70084-D04H-5

5 ug

Canine NRG1-­alpha Protein (EGF Domain, Fc Tag)

Proteins

Ca

Human

70084-D04H-100

100 ug

Canine NRG1-­alpha Protein (EGF Domain, Fc Tag)

Proteins

Ca

Human

70084-D04H-50

50 ug

Canine NRG1-­alpha Protein (EGF Domain, Fc Tag)

Proteins

Ca

Human

70084-D04H-10

10 ug

 

 

References

1

Nair P. (2005) Epidermal growth factor receptor family and its role in cancer progression. Curr. Sci. 88, 890-898.

 

2

Carpenter G, Cohen S (May 1990). "Epidermal growth factor". The Journal of Biological Chemistry. 265(14): 7709–12. PMID 2186024

 

3

Carpenter G, Cohen S. Epidermal growth factor. Annu Rev Biochem 1979; 48:193–216.

 

4

Franklin WA, Veve R, Hirsch FR, et al. Epidermal growth factor receptor family in lung cancer and premalignancy. Semin Oncol 2002; 29 (Suppl 4):3–14.

 

5

Herbst RS, Langer CJ. Epidermal growth factor receptors as a target for cancer treatment: The emerging role of IMC-C225 in the treatment of lung and head and neck cancer. Semin Oncol 2002;29:27–36.

 

6

Iliakis G. Cell cycle regulation in irradiated and nonirradiated cells. Semin Oncol 1997; 24:602–615.

 

7

Wakeling AE, Barker AJ, Daview DH, et al. Specific inhibition of epidermal growth factor receptor tyrosine kinase by 4-anilinoquinazolines. Breast Cancer Res Treat 1996;38:67– 73