M344

2 years -80 in solvent

MEL DS19 cells

100 nM [1], 100 nM [1], 100 nM [1], 100 nM [1], 100 nM [1], 100 nM [1]

72 hours

MEL DS19 cells (murine erythroleukemia cells) are maintained in D-MEM containing 100 units/mL penicillin G sodium and 100 ug/mL streptomycin sulfate supplemented with 10% fetal bovine serum at 37 C in a 5% CO2 atmosphere. To test M344 for potential to induce cell differentiation, log-phase cells with a population doubling time of 11 13 hours are used. Serial dilutions of M344 are prepared in 24-well plates using 1 mL of D-MEM/well. If M344 are dissolved in DMSO, control wells contains the same amount of solvent (generally 2 uL/mL of medium). Subsequently, the cell suspension is added to the wells. After 72 hours the experiment is evaluated. Cell numbers are counted using a Casy 1 TTC flow cytometer. The proliferation of treated cells is expressed as percent proliferation in comparison with the solvent control. Differentiated MEL cells accumulate hemoglobin. Therefore, the induction of cell differentiation is determined by benzidine staining according to the literature. To 100 uL of cell suspension is added 10 uL of a 0.4% solution of benzidine in 12% acetic acid containing 2% H2O2. Within 5 minutes hemoglobin-containing cells stains blue. Benzidine-positive and -negative cells are counted under the microscope in a hemocytometer, and the percentage of positive cells is calculated. M344 is first tested at 10 uM and 50 uM final concentration. According to activity/toxicity profile, a range of concentrations is chosen for a dose response analysis.

M344 is a potent HDAC inhibitor with IC50 of 100 nM and able to induce cell differentiation.

M344 is an inducer of terminal cell differentiation and a potent HDAC inhibitor.