EdU Cell Proliferation Image Kit (Orange Fluorescence)

Cell Proliferation EdU Image Kit (Orange Fluorescence) is a novel alternative to the BrdU assay. EdU (5-ethynyl-2´-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. Comparing to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation (typically using HCl or heat or digestion with DNase) for detection of the incorporated nucleoside. Detection is based on a click reaction, a copper-catalyzed covalent reaction between an azide and an alkyne, is complete within 30 minutes.

EdU

Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.

Abbkine Cell Proliferation EdU Image Kit (Orange Fluorescence) is a novel alternative to the BrdU assay.

• Optimized negative control is provided to rule out false positives
• No antibody needed.
• No denaturation steps, preservation of cell morphology and DNA integrity.
• Simple, reliable and time-saving than traditional methods.
• Proprietary AbFluor 545 azide (Ex/Em = 546/565 nm)-good photostability and minimizing fluorescence quenching.
• Optimized for fluorescent microscopy.