CheKine Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Colorimetric Assay Kit

Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) (EC 1.1.1.49) is a cytosolic enzyme that catalyzes the chemical reaction. This enzyme participates in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage from compounds like hydrogen peroxide.

Abbkine CheKine Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Colorimetric Assay Kit is designed for detecting G6PDH activity in the sample.

- Assay Buffer
- 6-Phosphogluconic acid
- NADP+

- 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
- Do not mix or substitute reagents or materials from other kit lots or vendors.
- The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
- Ensure all reagents and equipment are at the appropriate temperature before starting the assay.

Gel pack with blue ice.