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CheKine™ Micro Coenzyme Ⅰ NAD(H) Assay Kit

CheKine™ Micro Coenzyme Ⅰ NAD(H) Assay Kit

Item no.	ABK-KTB1020-96T
Manufacturer	Abbkine Scientific
Amount	96 T
Quantity options	48 T 96 T
Category	Cell Biology Cell Metabolism Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Shipping Condition	Cool pack
Available
Manufacturer - Type	Cell metabolism detection kit
Manufacturer - Applications	Abbkine CheKine NAD/NADH Assay Kit provides a convenient tool for sensitive detection of the NAD and NADH, and their ratio in various tissues and subcellular organelles. This Kit is based on enzyme cycling reaction (It does not recognize NADP+/NADPH), in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at OD565 nm, is proportionate to the NAD+ /NADH concentration in the sample.
Manufacturer - Category	Cell analysis
Manufacturer - Conjugate / Tag	NAD/NADH; NAD; NADH
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	Storage at –20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Background	Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP-ribosylation reactions and as a substrate for sirtuins.
Description	Abbkine CheKine™ Micro Coenzyme I NAD(H) Assay Kit is designed to detect the NAD and NADH, and their ratio in various tissues and cell.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	• Detect NAD+/NADH concentrations and ratios in serum, plasma, cell or plant/animal tissue extracts. • Detection limit of 0.78 µM and linearity up to 50 µM NAD+/NADH in 96-well plate assay.
Usage Notes	• Addition of Working Reagent should be quick and mixing should be brief but thorough. • The following substances interfere and should be avoided in sample preparation. EDTA (>0.5 mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%).
Kit components	• Assay Buffer • EtOH Solution • WST-8 Solution • Enhancer • NAD Cycling Enzyme Mix • NAD standard (10 mM) • NAD Extraction Buffer • NADH Extraction Buffer
Calibration range	0.78 µM-50 µM

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 T

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Delivery expected until 12/18/2025

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